Transcriptome in vivo analysis (TIVA) of spatially defined single cells in live tissue
A noninvasive method is reported for the isolation and profiling of the transcriptome from a single cell in the context of intact tissue. Transcriptome profiling of single cells resident in their natural microenvironment depends upon RNA capture methods that are both noninvasive and spatially precis...
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Published in | Nature methods Vol. 11; no. 2; pp. 190 - 196 |
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Main Authors | , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Nature Publishing Group US
01.02.2014
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | A noninvasive method is reported for the isolation and profiling of the transcriptome from a single cell in the context of intact tissue.
Transcriptome profiling of single cells resident in their natural microenvironment depends upon RNA capture methods that are both noninvasive and spatially precise. We engineered a transcriptome
in vivo
analysis (TIVA) tag, which upon photoactivation enables mRNA capture from single cells in live tissue. Using the TIVA tag in combination with RNA sequencing (RNA-seq), we analyzed transcriptome variance among single neurons in culture and in mouse and human tissue
in vivo.
Our data showed that the tissue microenvironment shapes the transcriptomic landscape of individual cells. The TIVA methodology is, to our knowledge, the first noninvasive approach for capturing mRNA from live single cells in their natural microenvironment. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1548-7091 1548-7105 |
DOI: | 10.1038/nmeth.2804 |