Identification of an E-box motif responsible for the expression of jasmonic acid-induced chitinase gene OsChia4a in rice

• Published in: Journal of plant physiology Vol. 169; no. 6; pp. 621 - 627
• Main Authors: Miyamoto, Koji, Shimizu, Takafumi, Lin, Fengqiu, Sainsbury, Frank, Thuenemann, Eva, Lomonossoff, George, Nojiri, Hideaki, Yamane, Hisakazu, Okada, Kazunori
• Format: Journal Article
• Language: English
• Published: Munich Elsevier GmbH 15.04.2012; Elsevier
• Subjects: Antifungal Agents - pharmacology; Base Pairing - genetics; Base Sequence; Basic helix-loop-helix transcription factor; Basic Helix-Loop-Helix Transcription Factors - genetics; Basic Helix-Loop-Helix Transcription Factors - metabolism; Biological and medical sciences; Chitinase; Chitinases - biosynthesis; Chitinases - genetics; Chitinases - pharmacology; Control; Cyclopentanes - pharmacology; Defence; Defence response; E-Box Elements - genetics; Enzyme Induction - drug effects; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant - drug effects; Genes; Genes, Plant - genetics; Germination; Jasmonic acid; Magnaporthe - drug effects; Microbial Sensitivity Tests; Molecular Sequence Data; Mutation - genetics; Oryza - cytology; Oryza - drug effects; Oryza - enzymology; Oryza - genetics; Oryza sativa; Oryza sativa L. cv. Nipponbare; Oxylipins - pharmacology; Plant physiology and development; Promoter Regions, Genetic - genetics; Proteins; Rice; Sequence Deletion; Translations; Chitinase; PR; Basic helix-loop-helix transcription factor; qRT-PCR; RLUC; bHLH; BSA; FLUC; Defence response; JA; Oryza sativa L. cv. Nipponbare; LUC; PCR; Jasmonic acid; Helix loop helix structure; Terpenoid; Monocotyledones; Enzyme; Identification; Defensive response; Cereal crop; Glycosylases; Oryza sativa; Gene; Plant physiology; Gramineae; Glycosidases; Angiospermae; Hydrolases; Plant growth substance; Spermatophyta; Transcription factor; Oryza sativa L cv. Nipponbare
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2011.12.008

The plant hormone jasmonic acid (JA) is known to be involved in multiple defence responses against pathogens, which include the production of pathogenesis-related (PR) proteins. In order to investigate the induction mechanism of the rice defence responses by JA, we performed transcriptome analyses and focused on a chitinase gene, OsChia4a, which was identified to be one of the highest JA-inductive genes. The recombinant protein of His-tagged OsChia4a exhibited an inhibitory effect against the spore germination and hyphal growth of Magnaporthe oryzae. The promoter analysis of OsChia4a revealed that the region from −515bp to −265bp upstream of the ATG translation initiation site was required for the responsiveness to JA. A subsequent mutation analysis indicated that an E-box (CANNTG) in this region act as a JA-responsive cis element. These results imply that a basic helix-loop-helix transcription factor is likely to be involved in the regulation of the OsChia4a expression in a JA-dependent manner.