Effect of mutation of C-terminal and heme binding region of Arabidopsis catalase on the import to peroxisomes

We evaluated the import of Arabidopsis catalase to peroxisomes under homogenous transient expression. The amino acids at −11 to −4 from the C-terminus are necessary for catalase import. The results are in agreement with the previous work under stable expression. We first demonstrate that heme-bindin...

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Published inBioscience, biotechnology, and biochemistry Vol. 83; no. 2; pp. 322 - 325
Main Authors Fujikawa, Yukichi, Suekawa, Marina, Endo, Satoshi, Fukami, Youjirou, Mano, Shoji, Nishimura, Mikio, Esaka, Muneharu
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 01.02.2019
Oxford University Press
Subjects
Arabidopsis - enzymology
Binding Sites
Catalase
Catalase - metabolism
Heme - metabolism
Heme-binding
Mutation
Peroxisomal protein import
Peroxisome targeting signal
Peroxisomes - metabolism
Protein Transport
Heme-binding
Peroxisomal protein import
Catalase
Peroxisome targeting signal
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Summary:We evaluated the import of Arabidopsis catalase to peroxisomes under homogenous transient expression. The amino acids at −11 to −4 from the C-terminus are necessary for catalase import. The results are in agreement with the previous work under stable expression. We first demonstrate that heme-binding sites are important for peroxisomal import, suggesting the importance of catalase folding. Abbreviations: AtCat: Arabidopsis catalase; PTS: peroxisomal targeting signal; PEX: Peroxin
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2018.1530094