Exhaustive Exercise Increases Spontaneous but Not fMLP-Induced Production of Reactive Oxygen Species by Circulating Phagocytes in Amateur Sportsmen

• Published in: Biology (Basel, Switzerland) Vol. 11; no. 1; p. 103
• Main Authors: Chmielecki, Adam, Bortnik, Krzysztof, Galczynski, Szymon, Padula, Gianluca, Jerczynska, Hanna, Stawski, Robert, Nowak, Dariusz
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10.01.2022; MDPI
• Subjects: Antioxidants; Blood; Chemiluminescence; cross-over studies; Enzymes; exercise; exhaustive exercise; Fitness equipment; Formyl peptides; granulocytes; Kinases; Neutrophils; Oxidants; peak oxygen uptake; Peptides; Phagocytes; Phosphorylation; Physiology; Proteins; Reactive oxygen species; Signal to noise ratio; Signal transduction; whole blood luminescence; granulocytes; exhaustive exercise; phagocytes; whole blood luminescence; reactive oxygen species
• ISSN: 2079-7737; 2079-7737
• DOI: 10.3390/biology11010103

Strenuous exercise alters the oxidative response of blood phagocytes to various agonists. However, little is known about spontaneous post exercise oxidant production by these cells. In this cross-over trial, we tested whether an exhaustive treadmill run at a speed corresponding to 70% of VO2max affects spontaneous and fMLP-provoked oxidant production by phagocytes in 18 amateur sportsmen. Blood was collected before, just after, and 1, 3, 5 and 24 h post exercise for determination of absolute and normalized per phagocyte count spontaneous (a-rLBCL, rLBCL) and fMLP-induced luminol-enhanced whole blood chemiluminescence (a-fMLP-LBCL, fMLP-LBCL). a-rLBCL and rLBCL increased by 2.5- and 1.5-times just after exercise (p < 0.05) and then returned to baseline or decreased by about 2-times at the remaining time-points, respectively. a-fMLP-LBCL increased 1.7- and 1.6-times just after and at 3 h post-exercise (p < 0.05), respectively, while fMLP-LBCL was suppressed by 1.5- to 2.3-times at 1, 3, 5 and 24 h post-exercise. No correlations were found between elevated post-exercise a-rLBCL, a-fMLP-LBCL and run distance to exhaustion. No changes of oxidants production were observed in the control arm (1 h resting instead of exercise). Exhaustive exercise decreased the blood phagocyte-specific oxidative response to fMLP while increasing transiently spontaneous oxidant generation, which could be a factor inducing secondary rise in antioxidant enzymes activity.