Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura

• Published in: Ecology and evolution Vol. 9; no. 19; pp. 11227 - 11231
• Main Authors: Le Chevalier, Hugo, Marí‐Mena, Neus, Carro, Belén, Prunier, Jérôme G., Bossu, Coralie, Darnet, Elodie, Souchet, Jérémie, Guillaume, Olivier, Calvez, Olivier, Bertrand, Romain, Barthe, Laurent, Pottier, Gilles, Martínez‐Sylvestre, Albert, Verdaguer‐Foz, Isabel, Mossoll‐Torres, Marc, Trochet, Audrey, Aubret, Fabien
• Format: Journal Article
• Language: English
• Published: Bognor Regis John Wiley & Sons, Inc 01.10.2019; Wiley Open Access; John Wiley and Sons Inc; Wiley
• Subjects: Alleles; Climate change; Deoxyribonucleic acid; DNA; Endangered & extinct species; Genetic diversity; Genetic markers; Genetic testing; Heterozygosity; Life Sciences; Linkage disequilibrium; Loci; Markers; Microsatellites; Mountains; Natrix maura; Original Research; polymorphic microsatellites; Polymorphism; Population; population genetics; viperine snake; Wildlife conservation; France; Natrix maura; population genetics; polymorphic microsatellites; viperine snake
• ISSN: 2045-7758; 2045-7758
• DOI: 10.1002/ece3.5623

Nineteen polymorphic microsatellite loci were identified and developed for Natrix maura. Polymorphism was assessed for 120 individuals sampled across four sampling sites from the French Pyrenees Mountains. The number of alleles per locus ranged from 3 to 15, and expected heterozygosity per locus ranged from 0.227 to 0.863. We tested for deviation from Hardy–Weinberg equilibrium and linkage disequilibrium and assessed the presence of null alleles for all loci, resulting in a selection of 14 high‐quality polymorphic markers. These markers will be extremely useful in identifying fine‐scale genetic structures and providing insight into conservation management plans of this species. Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura.