Synthesis and Degradation of the Major Allergens in Developing and Germinating Soybean Seed

• Published in: Journal of integrative plant biology Vol. 54; no. 1; pp. 4 - 14
• Main Authors: Wu, Yong‐Mei, Guan, Rong‐Xia, Liu, Zhang‐Xiong, Li, Run‐Zhi, Chang, Ru‐Zhen, Qiu, Li‐Juan
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Blackwell Publishing Asia 2012; National Key Facility of Gene Resources and Genetic Improvement/Key Laboratory of Crop,Germplasm Utilization, the Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China%National Key Facility of Gene Resources and Genetic Improvement/Key Laboratory of Crop,Germplasm Utilization, the Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China%Center of Agricultural Biotechnology, Shanxi Agricultural University, Taigu 030801, China; Center of Agricultural Biotechnology, Shanxi Agricultural University, Taigu 030801, China
• Subjects: allergen; allergens; Allergens - biosynthesis; Antigens, Plant - biosynthesis; cotyledons; flowering; food allergy; foods; Germination; Glycine max; Glycine max - metabolism; Glycoproteins - biosynthesis; imbibition; seed development; seed development and germination; seed germination; Seedlings - growth & development; Seeds - growth & development; Seeds - metabolism; soybean; Soybean Proteins - biosynthesis; soybeans; synthesis and degradation; 发展中国家; 发芽; 合成; 大豆种子; 甘氨酸; 过敏原; 过敏蛋白; 降解速度; soybean; allergen; food allergy; Glycine max; synthesis and degradation; seed development and germination
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/j.1744-7909.2011.01092.x

Gly m Bd 28K, Gly m Bd 30K and Gly m Bd 60K are the major soybean (Glycine max (L.) Merr.) allergens limiting the consumption of a good protein source for sensitive individuals. However, little is known about their temporal‐spatial expression during seed development and upon germination. The present data shows that soy allergens accumulated in both the embryonic axes and cotyledon, but expression patterns differed depending on the specific allergen. Allergens accumulated sooner and to a greater level in cotyledons than in embryonic axes. Gly m Bd 28 began at 14 d after flowering, 7 to 14 d earlier than Gly m Bd 30K and Gly m Bd 60K. Comparatively, their degradation was faster and more profound in embryonic axes than in cotyledons. Gly m Bd 60K began to decline at 36 h after imbibition and remained detectable up to 108 h in cotyledons. In contrast, the Glym Bd 60K protein was reduced at 24 h, and eventually disappeared at 96 h . In cotyledons Gly m Bd 28K first declined at 24 h, then increased from 36 h to 48 h, followed by its large reduction at 72 h after seed germination. These findings provide useful information on soy allergen biosynthesis and will help move forward towards developing a hypoallergenic soybean for safer food.