Improvement of anemia associated with chronic renal failure by recombinant human erythropoietin treatment in ICR-derived glomerulonephritis (ICGN) mice

• Published in: Journal of Veterinary Medical Science Vol. 66; no. 7; pp. 883 - 886
• Main Authors: Yamaguchi Yamada, M. (Kyoto Univ. (Japan)), Manabe, N, Goto, Y, Anan, S, Miyamoto, K, Miyamoto, Y, Nagao, M, Yamamoto, Y, Ogura, A
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.07.2004; Japan Science and Technology Agency
• Subjects: ANAEMIA; Anemia - drug therapy; Anemia - etiology; anemia associated with chronic renal failure; Animals; Bone Marrow - pathology; Disease Models, Animal; Erythrocyte Count; ERYTHROPOIETIN; Erythropoietin - therapeutic use; Female; GLOMERULONEPHRITIS; Glomerulonephritis - complications; Glomerulonephritis - drug therapy; Hematocrit; Hemoglobins; hereditary nephrotic mouse (ICGN); Injections, Subcutaneous; Kidney - pathology; Kidney Failure, Chronic - drug therapy; Kidney Failure, Chronic - etiology; Male; MICE; Mice, Inbred ICR; Recombinant Proteins; THERAPY
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.66.883

The ICR-derived glomerulonephritis (ICGN) mouse, a novel inbred mouse strain with a hereditary nephrotic syndrome, develops severe anemia associated with chronic renal failure. To reveal the pathogenic mechanism of anemia in ICGN mice, we subcutaneously administered recombinant human erythropoietin (rhEPO: 5 IU/mouse/day) or saline for 5 days to ICGN mice. In terminal-stage ICGN mice with severe anemia, rhEPO significantly increased hematocrit (Ht), red blood cells (RBC) and hemoglobin levels. Endogenous EPO levels in peripheral blood were reduced by rhEPO injection. No histopathological changes in bone marrow and kidneys were induced by rhEPO injection. Insufficiency of EPO may cause anemia in ICGN mice.