Enzyme assisted extraction of luteolin and apigenin from pigeonpea [Cajanuscajan (L.) Millsp.] leaves

Luteolin and apigenin are naturally occurring flavones with a wide spectrum of pharmacological properties. In the present study, enzyme assisted extraction of luteolin and apigenin from pigeonpea leaves using commercial plant cell wall degrading enzyme preparations including cellulase, beta-glucosid...

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Published inFood chemistry Vol. 111; no. 2; pp. 508 - 512
Main Authors Fu, Yu-Jie, Liu, Wei, Zu, Yuan-Gang, Tong, Mei-Hong, Li, Shuang-Ming, Yan, Ming-Ming, Efferth, Thomas, Luo, Hao
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 15.11.2008
[Amsterdam]: Elsevier Science
Elsevier
Subjects
Apigenin
Beta-glucosidase
Biological and medical sciences
Cajanus cajan
Cellulase
enzymatic treatment
Extraction
Food engineering
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
leaves
Luteolin
Pectinase
pigeon peas
plant extracts
Beta-glucosidase
Extraction
Luteolin
Apigenin
Cellulase
Pectinase
Flavones
Enzyme
Plant leaf
Polygalacturonase
Flavonoid
Glycosylases
Polyphenol
Plant part
Glycosidases
Hydrolases
Luteolin,Apigenin,Pectinase,Cellulase,Beta-glucosidase,Extraction
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Summary:Luteolin and apigenin are naturally occurring flavones with a wide spectrum of pharmacological properties. In the present study, enzyme assisted extraction of luteolin and apigenin from pigeonpea leaves using commercial plant cell wall degrading enzyme preparations including cellulase, beta-glucosidase and pectinase were examined. We found that pectinase offered a better performance in enhancement of the extraction yields of luteolin and apigenin than cellulase and beta-glucosidase. The pectinase assisted extraction process was further optimized by varying different parameters such as pectinase concentration, time of incubation, pH of pectinase solution, and incubation temperature. The optimum parameters were obtained as follows: 0.4mg/ml pectinase, incubation for 18h at 30–35°C, pH of pectinase solution 3.5–4. Under the optimum conditions, the extraction yields of luteolin and apigenin achieved 0.268 and 0.132mg/g in pectinase treated sample, which increased 248% and 239%, respectively, compared with the untreated ones.
Bibliography:http://dx.doi.org/10.1016/j.foodchem.2008.04.003
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2008.04.003