Inbreeding Alters the Gut Microbiota of the Banna Minipig

• Published in: Animals (Basel) Vol. 10; no. 11; p. 2125
• Main Authors: Wei, Limin, Zeng, Bo, Zhang, Siyuan, Li, Feng, Kong, Fanli, Ran, Haixia, Wei, Hong-Jiang, Zhao, Jiangchao, Li, Mingzhou, Li, Ying
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.11.2020; MDPI
• Subjects: 16S rRNA; Bacteria; Banna minipig inbred; Biological diversity; Biological research; Biology, Experimental; Biosynthesis; Composition; Deoxyribonucleic acid; Diannan small-ear pig; Discriminant analysis; DNA; Ear; Feces; Genes; Genetic aspects; Genetic diversity; Genetics; Genomes; Genomics; gut microbiota; Health aspects; Heterozygosity; Histidine; Hogs; Inbreeding; Intestinal microflora; Isoleucine; Klebsiella; Leucine; Metabolism; Metagenomics; Microbiomes; Microbiota; Microbiota (Symbiotic organisms); Offspring; Phenylalanine; PICRUSt2; Probiotics; rRNA 16S; Signal transduction; Swine; Taxonomy; Tryptophan; Tyrosine; Valine; China; Beijing China; United States > US
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani10112125

The gut microbiota coevolve with the host and can be stably transmitted to the offspring. Host genetics plays a crucial role in the composition and abundance of gut microbiota. Inbreeding can cause a decrease of the host’s genetic diversity and the heterozygosity. In this study, we used 16S rRNA gene sequencing to compare the differences of gut microbiota between the Diannan small-ear pig and Banna minipig inbred, aiming to understand the impact of inbreeding on the gut microbiota. Three dominant bacteria (Stenotrophlomonas, Streptococcus, and Lactobacillus) were steadily enriched in both the Diannan small-ear pig and Banna minipig inbred. After inbreeding, the gut microbiota alpha diversity and some potential probiotics (Bifidobacterium, Tricibacter, Ruminocaccae, Christensenellaceae, etc.) were significantly decreased, while the pathogenic Klebsiella bacteria was significantly increased. In addition, the predicted metagenomic analysis (PICRUSt2) indicated that several amino acid metabolisms (‘‘Valine, leucine, and isoleucine metabolism’’, ‘‘Phenylalanine, tyrosine, and tryptophan biosynthesis’’, ‘‘Histidine metabolism’’) were also markedly decreased after the inbreeding. Altogether our data reveal that host inbreeding altered the composition and the predicted function of the gut microbiome, which provides some data for the gut microbiota during inbreeding.