Influence of Laboratory Culture Media on in vitro Growth, Adhesion, and Biofilm Formation of Pseudomonas aeruginosa and Staphylococcus aureus

• Published in: Medical principles and practice Vol. 28; no. 1; pp. 28 - 35
• Main Authors: Wijesinghe, Gayan, Dilhari, Ayomi, Gayani, Buddhika , Kottegoda, Nilwala, Samaranayake, Lakshman, Weerasekera, Manjula
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01.04.2019
• Subjects: Bacteria; Biofilms; Biofilms - growth & development; Cell adhesion & migration; Culture Media; E coli; Humans; Laboratories; Microbiological Techniques - methods; Original Paper; Pathogens; Pseudomonas aeruginosa - growth & development; Staphylococcus aureus - growth & development; Staphylococcus infections; United States > US; Culture media; Pseudomonas aeruginosa; In vitro biofilm; Monospecies; Staphylococcus aureus; Dual species
• ISSN: 1011-7571; 1423-0151; 1423-0151
• DOI: 10.1159/000494757

Objective: Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilm infections are notoriously difficult to manage. This study aimed at investigating the influence of four different culture media on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa and S. aureus. Materials and Methods: We monitored four different culture media including Nutrient Broth, Brain Heart Infusion (BHI) broth, Luria-Bertani broth, and RPMI 1640 medium on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa (ATCC 27853) and S. aureus (ATCC 25923) using MTT assay and scanning electron microscopy (SEM). Results: The most robust growth of the mono- and dual-species cultures was noted in BHI broth. On the contrary, RPMI 1640 medium promoted maximal initial adhesion of both the mono- and dual-species, but BHI broth fostered the maximal biofilm growth. SEM images showed profuse extracellular polysaccharide production in biofilms, particularly in coculture, in BHI medium. Conclusion: Our data demonstrate that BHI broth, relative to the other tested media, is the most conducive for in vitro evaluation of biofilm and planktonic growth kinetics of these two pathogens, both in mono- and coculture.