Scale up of a novel tri-substrate fermentation for enhanced production of Aspergillus niger lipase for tallow hydrolysis

A novel tri-substrate fermentation (TSF) process was developed for the production of lipase from Aspergillus niger MTCC 2594 using agro-industrial residues, wheat bran (WB), coconut oil cake (COC) and an agro-product, wheat rawa (WR). The lipase activity was 628.7±13U/g dry substrate (U/gds) at 30°C...

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Published inBioresource technology Vol. 101; no. 17; pp. 6791 - 6796
Main Authors Edwinoliver, N.G., Thirunavukarasu, K., Naidu, R.B., Gowthaman, M.K., Kambe, T. Nakajima, Kamini, N.R.
Format Journal Article
LanguageEnglish
Published Kidlington Elsevier Ltd 01.09.2010
[New York, NY]: Elsevier Ltd
Elsevier
Subjects
Aspergillus niger
Aspergillus niger - enzymology
Biological and medical sciences
Biomass
Biotechnology
Fats - metabolism
Fermentation
Fundamental and applied biological sciences. Psychology
Hydrolysis
Lipase
Lipase - biosynthesis
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Scanning electron microscopy
Substrate Specificity
Tallow hydrolysis
Tri-substrate fermentation
Triticum aestivum
Tri-substrate fermentation
Scanning electron microscopy
Lipase
Aspergillus niger
Tallow hydrolysis
Enzyme
Fermentation
Fungi
Hydrolysis
Substrate
Tallow
Fungi Imperfecti
Lipolytic enzyme
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Summary:A novel tri-substrate fermentation (TSF) process was developed for the production of lipase from Aspergillus niger MTCC 2594 using agro-industrial residues, wheat bran (WB), coconut oil cake (COC) and an agro-product, wheat rawa (WR). The lipase activity was 628.7±13U/g dry substrate (U/gds) at 30°C and 96h and growth studies indicated that addition of WR significantly augmented the biomass and lipase production. Scale up of lipase production at 100g and 3kg (3×1kg) tray-level batch fermentation resulted in 96% and 83.0% of enzyme activities, respectively, at 72h. Maximum activity of 745.7±11U/gds was obtained, when fermented substrate was extracted in buffer containing 1% (w/v) sodium chloride and 0.5% (w/v) Triton X-100. Furthermore, the direct application of fermented substrate for tallow hydrolysis makes the process economical for industrial production of biofuel.
Bibliography:http://dx.doi.org/10.1016/j.biortech.2010.03.091
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2010.03.091