Optimization of the performance of the polymerase chain reaction in silicon-based microstructures

We have demonstrated the ability to perform real-time homogeneous, sequence specific detection of PCR products in silicon microstructures. Optimal design/processing result in equivalent performance (yield and specificity) for high surface-to-volume silicon structures as compared to larger volume rea...

Full description

Saved in:
Bibliographic Details
Published inNucleic acids research Vol. 25; no. 15; pp. 3164 - 3168
Main Authors Taylor, Theresa B., Winn-Deen, Emily S., Picozza, Enrico, Woudenberg, Timothy M., Albin, Michael
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.08.1997
Subjects
Actins - genetics
Polymerase Chain Reaction - instrumentation
Polymerase Chain Reaction - methods
Sensitivity and Specificity
Silicon
Online AccessGet full text

Cover

More Information
Summary:We have demonstrated the ability to perform real-time homogeneous, sequence specific detection of PCR products in silicon microstructures. Optimal design/processing result in equivalent performance (yield and specificity) for high surface-to-volume silicon structures as compared to larger volume reactions in polypropylene tubes. Amplifications in volumes as small as 0.5 µl and thermal cycling times reduced as much as 5-fold from that of conventional systems have been demonstrated for the microstructures.
Bibliography:istex:A3FFD6B2DEA8C6BDE3F43A48DF262550CCF2DC71
ark:/67375/HXZ-RGGDV4MV-0
Present address: Oncor Inc., 209 Perry Parkway, Gaithersburg, MD 20877, USA
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/25.15.3164