Optimization of the performance of the polymerase chain reaction in silicon-based microstructures
We have demonstrated the ability to perform real-time homogeneous, sequence specific detection of PCR products in silicon microstructures. Optimal design/processing result in equivalent performance (yield and specificity) for high surface-to-volume silicon structures as compared to larger volume rea...
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Published in | Nucleic acids research Vol. 25; no. 15; pp. 3164 - 3168 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
England
Oxford University Press
01.08.1997
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Subjects | |
Online Access | Get full text |
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Summary: | We have demonstrated the ability to perform real-time homogeneous, sequence specific detection of PCR products in silicon microstructures. Optimal design/processing result in equivalent performance (yield and specificity) for high surface-to-volume silicon structures as compared to larger volume reactions in polypropylene tubes. Amplifications in volumes as small as 0.5 µl and thermal cycling times reduced as much as 5-fold from that of conventional systems have been demonstrated for the microstructures. |
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Bibliography: | istex:A3FFD6B2DEA8C6BDE3F43A48DF262550CCF2DC71 ark:/67375/HXZ-RGGDV4MV-0 Present address: Oncor Inc., 209 Perry Parkway, Gaithersburg, MD 20877, USA ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0305-1048 1362-4962 1362-4962 |
DOI: | 10.1093/nar/25.15.3164 |