Microbiological reduction of chromium(VI) in presence of pyrolusite-coated sand by Shewanella alga Simidu ATCC 55627 in laboratory column experiments

• Published in: Chemosphere (Oxford) Vol. 52; no. 1; pp. 175 - 183
• Main Authors: Guha, Hillol, Jayachandran, Krishnaswamy, Maurrasse, Florentin
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.07.2003; Elsevier
• Subjects: Adsorption; Biodegradation of pollutants; Biodegradation, Environmental; Biological and medical sciences; Biotechnology; BrY-MT; Carcinogens, Environmental - metabolism; Chromium - metabolism; Column experiments; Environment and pollution; Fundamental and applied biological sciences. Psychology; Hexavalent chromium; Industrial applications and implications. Economical aspects; Manganese Compounds - chemistry; Mn-oxides; Oxides - chemistry; pyrolusite; Redox reactions; Shewanella; Shewanella - physiology; Shewanella alga; Silicon Dioxide - chemistry; Soil Pollutants - metabolism; Hexavalent chromium; Mn-oxides; Column experiments; BrY-MT; Redox reactions; Pyrolusite; Oxidation reduction; Shewanella; Pollutant; Reduction; Chromium IV; Biochemical reaction; Bacteria; Vibrionaceae; Bioremediation
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/S0045-6535(03)00104-8

Hexavalent chromium (Cr(VI)) was reduced to non-toxic trivalent chromium (Cr(III)) by a dissimilatory metal reducing bacteria, Shewanella alga Simidu (BrY-MT) ATCC 55627. A series of dynamic column experiments were conducted to provide an understanding of Cr(VI) reduction by the facultative anaerobe BrY-MT in the presence of pyrolusite (β-MnO2) coated sand and uncoated-quartz sand. All dynamic column experiments were conducted under growth conditions using Cr(VI) as the terminal electron acceptor and lactate as the electron donor and energy source. Reduction of Cr(VI) was rapid (within 8 h) in columns packed with uncoated quartz sand and BrY-MT, whereas Cr(VI) reduction by BrY-MT was delayed (57 h) in the presence of β-MnO2-coated sand. The role of β-MnO2 in this study was to provide oxidation of trivalent chromium (Cr(III)). BrY-MT attachment was higher on β-MnO2-coated sand than on uncoated quartz sand at 10, 60, and 85.5 h. Results have shown that this particular strain of Shewanella did not appreciably reduce Mn(IV) to Mn(II) species nor biosorbed Cr and Mn during its metabolic activities.