In situ tissue regeneration using a novel tissue-engineered, small-caliber vascular graft without cell seeding

• Published in: The Journal of thoracic and cardiovascular surgery Vol. 136; no. 4; pp. 900 - 907
• Main Authors: Yokota, Takenori, MD, Ichikawa, Hajime, MD, PhD, Matsumiya, Goro, MD, PhD, Kuratani, Toru, MD, PhD, Sakaguchi, Taichi, MD, PhD, Iwai, Shigemitsu, MD, PhD, Shirakawa, Yukitoshi, MD, PhD, Torikai, Kei, MD, PhD, Saito, Atsuhiro, PhD, Uchimura, Eiichiro, PhD, Kawaguchi, Naomasa, PhD, Matsuura, Nariaki, MD, PhD, Sawa, Yoshiki, MD, PhD
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Mosby, Inc 01.10.2008; AATS/WTSA; Elsevier
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Biological and medical sciences; Bioprosthesis; Blood Vessel Prosthesis; Blood Vessel Prosthesis Implantation - adverse effects; Blood Vessel Prosthesis Implantation - methods; Cardiology. Vascular system; Cardiothoracic Surgery; Carotid Arteries - pathology; Carotid Arteries - surgery; Carotid Stenosis - surgery; Disease Models, Animal; Dogs; Graft Rejection; Graft Survival; Immunohistochemistry; Lactic Acid - chemistry; Medical sciences; Pneumology; Polyesters; Polyglycolic Acid - chemistry; Polymers - chemistry; Random Allocation; Regeneration - physiology; Sensitivity and Specificity; Tissue Engineering; Vascular Patency - physiology; 33; PLLA; TEVG; poly-L-lactic acid; expanded polytetrafluoroethylene; PGA; ePTFE; polyglycolic acid; tissue-engineered vascular graft; ECM; extracellular matrix; In situ; Tissue culture; Seeding; Regeneration; Tissue; Treatment; Surgery; Blood vessel; Anesthesia; Graft; Circulatory system; Cardiology; Cell
• ISSN: 0022-5223; 1097-685X
• DOI: 10.1016/j.jtcvs.2008.02.058

Objective Various types of natural and synthetic scaffolds with arterial tissue cells or differentiated stem cells have recently attracted interest as potential small-caliber vascular grafts. It was thought that the synthetic graft with the potential to promote autologous tissue regeneration without any seeding would be more practical than a seeded graft. In this study, we investigated in situ tissue regeneration in small-diameter arteries using a novel tissue-engineered biodegradable vascular graft that did not require ex vivo cell seeding. Methods Small-caliber vascular grafts (4 mm in diameter) were fabricated by compounding a collagen microsponge with a biodegradable woven polymer tube that was constructed in a plain weave pattern with a double layer of polyglycolic acid (core) and poly-L-lactic acid (sheath) fibers. We implanted these tissue-engineered vascular grafts bilaterally into the carotid arteries of mongrel dogs (body weight, 20–25 kg). No anticoagulation regimen was used after implantation. We sacrificed the dogs 2, 4, 6, and 12 months (n = 4 in each group) after implantation and evaluated the explants histologically and biochemically. Results All of the tissue-engineered vascular grafts were patent with no signs of thrombosis or aneurysm at any time. Histologic and biochemical examinations showed excellent in situ tissue regeneration with an endothelial cell monolayer, smooth muscle cells, and a reconstructed vessel wall with elastin and collagen fibers. Conclusion Our study indicated that this novel tissue-engineered vascular graft promoted in situ tissue regeneration and did not require ex vivo cell seeding, thereby conferring better patency on small-caliber vascular prostheses.