Developmental and cell cycle regulation of alfalfa nucMs1, a plant homolog of the yeast Nsr1 and mammalian nucleolin

• Published in: The Plant cell Vol. 8; no. 3; pp. 417 - 428
• Main Authors: Bogre, L. (University of Vienna, Vienna, Austria.), Jonak, C, Mink, M, Meskiene, I, Traas, J, Dang, T.C.H, Swoboda, I, Plank, C, Wagner, E, Heberle-Bors, E
• Format: Journal Article
• Language: English
• Published: England American Society of Plant Physiologists 01.03.1996; American Society of Plant Biologists (ASPB)
• Subjects: ADN; Alfalfa; Animals; ARN MENSAJERO; ARN MESSAGER; Base Sequence; BIOCHIMIE; BIOQUIMICA; BOURGEON; Cell cycle; Cell Cycle - physiology; Cell Cycle Proteins - biosynthesis; Cell Cycle Proteins - metabolism; Cell Division; Cell growth; Cell nucleolus; Cell Nucleolus - physiology; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; DIVISION CELLULAIRE; DIVISION CELULAR; EXPRESION GENICA; EXPRESSION DES GENES; FEUILLE; FLEUR; FLORES; Fungal Proteins - metabolism; GENE; Gene Library; GENES; Genetics; Histones; HOJAS; Homeostasis; Life Sciences; Mammals; MEDICAGO SATIVA; Medicago sativa - genetics; Medicago sativa - physiology; MERISTEMAS; MERISTEME; METHODE; METODOS; Molecular Sequence Data; Nuclear Proteins - biosynthesis; Nuclear Proteins - metabolism; NUCLEOLE; Nucleolin; NUCLEOLOS; NUCLEOPROTEINAS; NUCLEOPROTEINE; Oligodeoxyribonucleotides; Phosphoproteins - biosynthesis; Phosphoproteins - metabolism; Plant cells; Plant Proteins - biosynthesis; Plant Proteins - metabolism; Plants genetics; POLLO; POULET; Proteins; RACINE; RAICES; RNA; RNA-Binding Proteins; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - physiology; Saccharomyces cerevisiae Proteins; SECUENCIA NUCLEICA; SEQUENCE NUCLEIQUE; Transcription, Genetic; Yeasts; YEMA (PLANTA)
• ISSN: 1040-4651; 1532-298X
• DOI: 10.1105/tpc.8.3.417

We report here the isolation and characterization of the nucMs1 alfalfa cDNA, whose predicted amino acid sequence structurally resembles the yeast Nsr1 protein and animal nucleolins. Those proteins consist of an N-terminal acidic domain, centrally located RNA recognition motifs (RRMs), and a C-terminal glycine- and arginine-rich domain. In comparison with animal nucleolins that contain four RRMs, NucMs1 more closely resembles the yeast Nsr1 protein, which contains only two RRMs. A NucMs1 C-terminal peptide antibody specifically recognized a 95-kD nucleolar protein in alfalfa cells that changed its localization in a cell cycle-dependent manner. The nucMs1 transcript and p95nucMs1 protein levels correlated with cell proliferation, and nucMs1 gene expression wee found to be induced in the G1 phase upon mitogenic stimulation of G0-arrested leaf cells. In situ hybridization analysis of different alfalfa organs during various developmental stages showed that nucMs1 gene expression is highest in root meristematic cells, but it is also found in other meristematic cells of the plant body. nucMs1 expression is tightly linked to cell proliferation but does not depend on a particular cell cycle phase. No nucMs1 expression was observed in cells that had exited the cell cycle and were undergoing differentiation or polar growth, indicating that nucMs1 may not be necessary for processes other than cell proliferation