Electrophysiological basis of metabolic-syndrome-induced cardiac dysfunction

• Published in: Canadian journal of physiology and pharmacology Vol. 94; no. 10; pp. 1064 - 1073
• Main Authors: Okatan, Esma N, Durak, Aysegul Toy, Turan, Belma
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.10.2016; Canadian Science Publishing NRC Research Press
• Subjects: Analysis; Calcium ions; Cardiomyocytes; Cardiovascular disease; Kinases; Metabolic diseases; Metabolic disorders; Sarcoplasmic reticulum; Turkey; résistance à l’insuline; high sucrose diet; fonction cardiaque; heart function; SERCA; calcium transients; régime à haute teneur en sucrose; diabète; courants calciques transitoires; calcium sparks; étincelles calciques; diabetes; insulin resistance
• ISSN: 0008-4212; 1205-7541
• DOI: 10.1139/cjpp-2015-0531

Myocardial contractility is controlled by intracellular Ca cycling with the contribution of sarcoplasmic reticulum (SR). In this study, we aimed to investigate the role of altered SR function in defective regulation of intracellular Ca levels in rats with metabolic syndrome (MetS) induced by a 16-week high-sucrose drinking-water diet. Electric-field stimulated transient intracellular Ca changes in MetS cardiomyocytes exhibited significantly reduced amplitude (∼30%) and prolonged time courses (2-fold), as well as depressed SR Ca loading (∼55%) with increased basal Ca level. Consistent with these data, altered ryanodine receptor (RyR2) function and SERCA2a activity were found in MetS cardiomyocytes through Ca spark measurements and caffeine application assay in a state in which sodium calcium exchanger was inhibited. Furthermore, tetracaine application assay results and hyperphosphorylated level of RyR2 also support the "leaky RyR2" hypothesis. Moreover, altered phosphorylation levels of phospholamban (PLN) support the depressed SERCA2a-activity thesis and these alterations in the phosphorylation of Ca -handling proteins are correlated with altered protein kinase and phosphatase activity in MetS cardiomyocytes. In conclusion, MetS-rat heart exhibits altered Ca signaling largely due to altered SR function via changes in RyR2 and SERCA2a activity. These results point to RyR2 and SERCA2a as potential pharmacological targets for restoring intracellular Ca homeostasis and, thereby, combatting dysfunction in MetS-rat heart.