Glutathione S-Transferases and Cytochrome P450 Enzyme Expression in Patients with Intracranial Tumors: Preliminary Report of 55 Patients

• Published in: Medical principles and practice Vol. 28; no. 1; pp. 56 - 62
• Main Authors: Kural, Cahit, Kaya Kocdogan, Arzu, Şimşek, Gulcin Güler, Oğuztüzün, Serpil, Kaygın, Pınar, Yılmaz, Irmak, Bayram, Tugbag, Izci, Yusuf
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01.04.2019
• Subjects: Adolescent; Adult; Aged; Biomarkers; Biomarkers - metabolism; Brain cancer; Brain Neoplasms - enzymology; Brain Neoplasms - pathology; Brain research; Breasts; Cancer therapies; Chemotherapy; Child; Cytochrome; Cytochrome P-450 CYP1A1 - metabolism; Cytochrome P-450 CYP1B1 - metabolism; Cytochrome P-450 Enzyme System - metabolism; Deoxyribonucleic acid; DNA; DNA methylation; Enzymes; Female; Glutathione S-Transferase pi - metabolism; Glutathione Transferase - metabolism; Humans; Male; Medical prognosis; Metabolism; Middle Aged; Original Paper; Patients; Prostate; Tumors; Turkey; Young Adult; Turkey; Tumor biology; Brain; Glutathione-S-transferase; Cytochrome p450
• ISSN: 1011-7571; 1423-0151
• DOI: 10.1159/000494496

Objective: Intracranial tumors are one of the most frightening and difficult-to-treat tumor types. In addition to surgery, protocols such as chemotherapy and radiotherapy also take place in the treatment. Glutathione S-transferase (GST) and cytochrome P450 (CYP) enzymes are prominent drug-metabolizing enzymes in the human body. The aim of this study is to show the expression of GSTP1, GSTM1, CYP1A1, and CYP1B1 in different types of brain tumors and compare our results with those in the literature. Subjects and Methods: The expression of GSTP1, GSTM1, CYP1A1, and CYP1B1 was analyzed using immunostaining in 55 patients with intracranial tumors in 2016–2017. For GST and CYP expression in normal brain tissue, samples of a portion of surrounding normal brain tissue as well as a matched far neighbor of tumor tissue were used. The demographic features of the patients were documented and the expression results compared. Results: The mean age of the patients was 46.72 years; 29 patients were female and 26 were male. Fifty-seven specimens were obtained from 55 patients. Among them, meningioma was diagnosed in 12, metastases in 12, glioblastoma in 9, and pituitary adenoma in 5. The highest GSTP1, GSTM1, and CYP­1A1 expressions were observed in pituitary adenomas. The lowest GSTP1 expression was detected in glioblastomas and the lowest CYP1B1 expression in pituitary adenomas. Conclusion: GSTP1 and CYP expression is increased in intracranial tumors. These results should be confirmed with a larger series and different enzyme subtypes.