Enhanced spectrofluorimetric determination of esomeprazole and pantoprazole in dosage forms and spiked human plasma using organized media

• Published in: Luminescence (Chichester, England) Vol. 30; no. 3; pp. 343 - 351
• Main Authors: Belal, Fathalla, Sharaf EL-Din, Mohie, Tolba, Manar M., Alaa, Heba
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.05.2015; Wiley Subscription Services, Inc
• Subjects: 2-Pyridinylmethylsulfinylbenzimidazoles - analysis; 2-Pyridinylmethylsulfinylbenzimidazoles - blood; Capsules - analysis; Dosage; Drugs; esomeprazole; Esomeprazole - analysis; Esomeprazole - blood; Fluorescence; Human; human plasma; Humans; Hydrogen-Ion Concentration; In vitro testing; Limit of Detection; Linearity; Methyl alcohol; Methylcellulose - chemistry; micelle-enhanced; pantoprazole; Reproducibility of Results; Sodium dodecyl sulfate; Sodium Dodecyl Sulfate - chemistry; Solvents - chemistry; Spectra; spectrofluorimetry; Spectrometry, Fluorescence - methods; Tablets - analysis; pantoprazole; spectrofluorimetry; micelle-enhanced; esomeprazole; human plasma
• ISSN: 1522-7235; 1522-7243
• DOI: 10.1002/bio.2737

A simple, sensitive and rapid spectrofluorimetric method was developed for the determination of esomeprazole (EMZ) and pantoprazole (PRZ) in their pharmaceutical formulations and human plasma. The proposed method is based on the fluorescence spectral behavior of EMZ in methanol in the presence of 0.1 m NaOH containing 0.5% methyl cellulose (MC) at 306/345 nm. The fluorescence intensity of EMZ was enhanced about 1.3‐fold and good linearity in the range 0.4–4.0 µg/mL with a lower detection limit of 0.04 µg/mL and lower quantification limit of 0.14 µg/mL. For PRZ, its methanolic solution exhibited marked native fluorescence at 290/325 nm after enhancement (about 2.1‐ or 1.4‐fold) using either 0.025% sodium dodecyl sulfate (SDS) or 0.05% MC in the presence of 0.2 m borate buffer of pH 9.5. The fluorescence–concentration plots of PRZ were rectilinear over the ranges 0.2–2.0 and 0.3–3.0 µg/mL with lower detection limits of 0.02 and 0.03 µg/mL and lower quantification limits of 0.07 and 0.09 µg/mL using sodium dodecyl sulfate and MC, respectively. The method was successfully applied to the analysis of EMZ and PRZ in their commercial dosage forms and the results were in good agreement with those obtained with the comparison method. Furthermore, in a preliminary investigation, the proposed method was extended to the in vitro determination of the two drugs in spiked human plasma and the results were satisfactory. Copyright © 2014 John Wiley & Sons, Ltd.