A New Method for the Assay of Purine Metabolic Enzymes

We have established new colorimetric methods for the assay of adenosine deaminase, purine nucleoside phosphorylase and guanase activities in serum, based on the formation of hydrogen peroxide with xanthine oxidase as a coupling enzyme. [chemical formula] The proposed methods were found to be precise...

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Published inChemical & pharmaceutical bulletin Vol. 29; no. 2; pp. 426 - 429
Main Authors SUGIURA, MAMORU, KATO, KENJI, ADACHI, TETSUO, ITO, YOSHIMASA, HIRANO, KAZUYUKI, SAWAKI, SHUNJI
Format Journal Article
LanguageEnglish
Published Japan The Pharmaceutical Society of Japan 1981
Japan Science and Technology Agency
Subjects
adenosine deaminase
Adenosine Deaminase - blood
Aminohydrolases - blood
assays
colorimetric method
guanine deaminase
Guanine Deaminase - blood
Humans
Methods
Nucleoside Deaminases - blood
Pentosyltransferases - blood
purine-nucleoside phosphorylase
Purine-Nucleoside Phosphorylase - blood
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Summary:We have established new colorimetric methods for the assay of adenosine deaminase, purine nucleoside phosphorylase and guanase activities in serum, based on the formation of hydrogen peroxide with xanthine oxidase as a coupling enzyme. [chemical formula] The proposed methods were found to be precise and convenient. Under the assay conditions, the mean levels of adenosine deaminase, purine nucleoside phosphorylase and guanase activities in the sera of normal subjects were 5.8±2.2 I. U./1, 3.7±2.1 I. U./1 and 0.5±0.3 I. U./1, respectively.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.29.426