Variation of Bacterial and Archaeal Community Structures in a Full-Scale Constructed Wetlands for Wastewater Treatment

• Published in: Archaea (Vancouver) Vol. 2018; no. 2018; pp. 1 - 12
• Main Authors: Wang, Xin, Song, Zhi-wen, Xu, Ai-ling, Chen, Xiang, Lang, Xiu-lu, Wang, He-bing
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 2018; Hindawi; John Wiley & Sons, Inc; Hindawi Limited; Wiley
• Subjects: Abundance; Ammonia; Analysis; Archaea; Archaea - classification; Archaea - growth & development; Archaea - isolation & purification; Artificial wetlands; Bacteria; Bacteria - classification; Bacteria - growth & development; Bacteria - isolation & purification; Biota; Cluster Analysis; Communities; Composition; Constructed wetlands; Construction; Deoxyribonucleic acid; DNA; DNA, Archaeal - chemistry; DNA, Archaeal - genetics; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Dominant species; Flora; Free surfaces; Influents; Microorganisms; Phylogeny; Plants; Pollutants; Population Dynamics; Purification; Relative abundance; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Sequences; Sewage; Software; Soil; Soil microbiology; Soil structure; Soil water; Soils; Surface flow; Waste Water - microbiology; Wastewater; Wastewater treatment; Water Purification - methods; Water treatment; Wetlands; China; Germany
• ISSN: 1472-3646; 1472-3654
• DOI: 10.1155/2018/9319345

Microorganisms play important roles in the reduction of organic and inorganic pollutants in constructed wetlands used for the treatment of wastewater. However, the diversity and structure of microbial community in constructed wetland system remain poorly known. In this study, the Illumina MiSeq Sequencing of 16S rDNA was used to analyze the bacterial and archaeal microbial community structures of soil and water in a free surface flow constructed wetland, and the differences of bacterial communities and archaeal compositions between soil and water were compared. The results showed that the Proteobacteria were the dominant bacteria, making up 35.38%~48.66% relative abundance. Euryarchaeotic were the absolute dominant archaea in the influent sample with the relative abundance of 93.29%, while Thaumarchaeota showed dominance in the other three samples, making up 50.58%~75.70%. The relative abundances of different species showed great changes in bacteria and archaea, and the number of dominant species in bacteria was much higher than that in archaea. Compared to archaea, the community compositions of bacteria were more abundant and the changes were more significant. Meanwhile, bacteria and archaea had large differences in compositions between water and soil. The microbial richness in water was significantly higher than that in soil. Simultaneously, soil had a significant enrichment effect on some microbial flora.