Addition of MCP-1 and MIP-3β to the IL-8 appraisal in peritoneal fluid enhances the probability of identifying women with endometriosis

• Published in: Journal of reproductive immunology Vol. 109; pp. 66 - 73
• Main Authors: Borrelli, G.M., Kaufmann, A.M., Abrão, M.S., Mechsner, S.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.06.2015
• Subjects: Adult; Ascitic Fluid - metabolism; Biomarkers; Chemokine CCL19 - metabolism; Chemokine CCL2 - metabolism; Chemokines; Endometriosis; Endometriosis - metabolism; Female; Follicular Phase; Humans; Interleukin-8 - metabolism; Luteal Phase; Obstetrics and Gynecology; Panel of markers; Retrospective Studies; Biomarkers; Endometriosis; Panel of markers; Chemokines
• ISSN: 0165-0378; 1872-7603; 1872-7603
• DOI: 10.1016/j.jri.2015.01.003

•Several chemokines have been tested for endometriosis so far.•For the first time the systematically selected chemokines were evaluated together in a multiplexed assay.•IL-8, MCP-1, and MIP-3β had statistically significant higher concentrations in the PF of women with endometriosis.•The combined assessment of MCP-1, MIP-3β, and IL-8 improved the likelihood of identifying patients with endometriosis. Chemokines have been associated with endometriosis. Our study was aimed at evaluating the levels of six chemokines – CXCL8 (IL-8), CXCL12 (SDF-1), CCL2 (MCP-1), CCL5 (RANTES), CCL19 (MIP-3β), and CCL21 (6-Ckine) – in the peritoneal fluid (PF) of patients with and controls without endometriosis by multiplexed cytokine assay. In this retrospective case–control study conducted at the Charité University Hospital, patients (n=36) and controls (n=27) were enrolled. The patients were separated into groups according to stage of the disease: I–II (n=21), III–IV (n=15), and according to clinical findings: peritoneal endometriosis (PE; n=7), deep-infiltrating endometriosis (DIE) affecting the retrocervical area (n=13) or the bowel/rectovaginal site (n=14). The subjects were also separated according to the cycle phase: follicular (n=14) or luteal (n=8) and the previous use (n=25) or not (n=38) of hormones. PF was collected from all subjects (n=63) consecutively during laparoscopy. The concentration of chemokines in the PF was assessed using Luminex® x-MAP® technology. Sensitivity and specificity were calculated. A model of multiple logistic regressions estimated the odds of endometriosis for each combination of the chemokines detected. We observed significantly higher concentrations of IL-8 (p<0.001), MCP-1 (p=0.014), and MIP-3β (p=0.022) in the PF of women with endometriosis than in the controls. A joint evaluation revealed that elevated levels of the three chemokines had a positive endometriosis prediction value of 89.1%. The combined assessment of MCP-1, MIP-3β, and IL-8 concentration in PF improved the likelihood of identifying patients with endometriosis. Future studies should investigate this panel in peripheral blood samples.