Leukocyte Cell Population Analysis From the Coulter Automatic Blood Cell Analyzer DxH800 to Monitor the Effect of G-CSF

• Published in: Journal of clinical laboratory analysis Vol. 26; no. 3; pp. 194 - 199
• Main Authors: Lee, Sung-Eun, Lim, Jihyang, Kim, Yonggoo, Min, Woo-Sung, Han, Kyungja
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.05.2012; John Wiley & Sons, Inc; John Wiley and Sons Inc
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Blood cells; Case-Control Studies; Cellular biology; Female; granulocyte colony-stimulating factor; Granulocyte Colony-Stimulating Factor - pharmacology; Humans; leukocyte cell population; Leukocyte Count - methods; Leukocytes; Male; Middle Aged; Neutrophils - cytology; Neutrophils - drug effects; ROC Curve; Sensitivity and Specificity; toxic granulation neutrophils
• ISSN: 0887-8013; 1098-2825; 1098-2825
• DOI: 10.1002/jcla.21493

Background Granulocyte colony‐stimulating factor (G‐CSF) induces the formation of toxic granulation neutrophils (TGNs), which are found in many inflammatory responses. Cell population data (CPD) may be able to clarify the effect of G‐CSF, and potentially help doctors in discriminating the effect of G‐CSF from other inflammatory situations. Methods To achieve this, we performed analyses of leukocyte CPD from normal controls and healthy donors that had received G‐CSF for peripheral blood stem cells (PBSCs) mobilization (G‐CSF group). Results Two hundred and seventy‐one subjects were enrolled as normal controls, and 21 subjects were enrolled in the G‐CSF group. Mean volume (MN‐V)‐neutrophils (NE), mean axial light loss (MN‐AL2)‐NE, and all standard deviation (SD) parameters increased significantly, whereas all light scattering parameters, mean median angle light scatter (MN‐MALS)‐NE, mean upper median angle light scatter (MN‐UMALS)‐NE, mean lower median angle light scatter (MN‐LMALS)‐NE, and mean low angle light scatter (MN‐LALS)‐NE reduced significantly in the G‐CSF group. MN‐V‐lymphocytes (LY) from the G‐CSF group showed no significant difference (P = 0.143), whereas MN‐V‐monocytes (MO) were significantly decreased (P < 0.001). Receiver operating characteristic (ROC) curves for the discrimination of the G‐CSF group from normal controls showed excellent sensitivity in SD‐LALS‐NE (at 30.85, sensitivity 95.2%, specificity 76.0%), MN‐AL2‐NE (at 134.5, sensitivity 90.5%, specificity 83.0%), and SD‐AL2‐NE (at 16.4, sensitivity 95.2%, specificity 95.2). Several CPD parameters of lymphocytes and monocytes, as well as neutrophils can be used as markers for determining the effect of G‐CSF. Conclusion Our data show that many CPD of leukocytes can be considered to be useful parameters of the effect of G‐CSF. J. Clin. Lab. Anal. 26:194‐199, 2012. © 2012 Wiley Periodicals, Inc.