Proinflammatory microenvironment promotes lymphoma progression in mice with high megakaryocyte and TPO levels

• Published in: Blood advances Vol. 7; no. 8; pp. 1560 - 1571
• Main Authors: Au, Amanda E., Corbin, Jason, Lebois, Marion, Gangatirkar, Pradnya, Yassinson, Fatme, Hyslop, Stephanie R., Cannon, Ping, Mason, Kylie D., Li-Wai-Suen, Connie S. N., Garnham, Alexandra L., Moujalled, Diane, Cimmino, Luisa, Alexander, Warren S., Josefsson, Emma C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 25.04.2023; The American Society of Hematology
• Subjects: Animals; Blood Platelets - metabolism; Hematologi; Hematology; Humans; Lymphoid Neoplasia; Lymphoma - genetics; Megakaryocytes - metabolism; Mice; Receptors, Thrombopoietin; Thrombocytopenia - genetics; Tumor Microenvironment
• ISSN: 2473-9529; 2473-9537
• DOI: 10.1182/bloodadvances.2022007849

•Increased levels of interleukin 1 were found in the bone marrow fluid of TpoTg mice, whereas levels were lowered in Mpl−/− mice.•A proinflammatory microenvironment promoted Eμ-myc lymphoma progression in TpoTg mice with high megakaryocyte and thrombopoietin levels. [Display omitted] Platelets have been shown to enhance the survival of lymphoma cell lines. However, it remains unclear whether they play a role in lymphoma. Here, we investigated the potential role of platelets and/or megakaryocytes in the progression of Eμ-myc lymphoma. Eμ-myc tumor cells were transplanted into recipient wild-type (WT) control, Mpl−/−, or TpoTg mice, which exhibited normal, low, and high platelet and megakaryocyte counts, respectively. TpoTg mice that underwent transplantation exhibited enhanced lymphoma progression with increased white blood cell (WBC) counts, spleen and lymph node weights, and enhanced liver infiltration when compared with WT mice. Conversely, tumor-bearing Mpl−/− mice had reduced WBC counts, lymph node weights, and less liver infiltration than WT mice. Using an Mpl-deficient thrombocytopenic immunocompromised mouse model, our results were confirmed using the human non-Hodgkin lymphoma GRANTA cell line. Although we found that platelets and platelet-released molecules supported Eμ-myc tumor cell survival in vitro, pharmacological inhibition of platelet function or anticoagulation in WT mice transplanted with Eμ-myc did not improve disease outcome. Furthermore, transient platelet depletion or sustained Bcl-xL–dependent thrombocytopenia did not alter lymphoma progression. Cytokine analysis of the bone marrow fluid microenvironment revealed increased levels of the proinflammatory molecule interleukin 1 in TpoTg mice, whereas these levels were lower in Mpl−/− mice. Moreover, RNA sequencing of blood-resident Eμ-myc lymphoma cells from TpoTg and WT mice after tumor transplantation revealed the upregulation of hallmark gene sets associated with an inflammatory response in TpoTg mice. We propose that the proinflammatory microenvironment in TpoTg mice promotes lymphoma progression.