Simultaneous Determination of Lead and Cadmium in Biological Samples by Multichannel Graphite Furnace Atomic Absorption Spectrometry

• Published in: Eisei kagaku Vol. 37; no. 5; pp. 401 - 404
• Main Authors: IMAI, SHOJI, HAYASHI, YASUHISA, SAITO, KENGO, ISHIKURA, HIROSHI, TANAKA, TOSHIYUKI
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1991; 公益社団法人日本薬学会; Nippon Yakugakkai
• Subjects: ammonium dihydrogen phosphate; Biological and medical sciences; blood; cadmium; Chemical and industrial products toxicology. Toxic occupational diseases; graphite furnace atomic absorption spectrometry; lead; Medical sciences; Metals and various inorganic compounds; serum; simultaneous determination; Toxicology; Assay; Human; Cadmium; Graphite furnace; Concurrent; Biological medium; Lead; Serum; Heavy metal; Atomic absorption spectrometry
• ISSN: 0013-273X
• DOI: 10.1248/jhs1956.37.401

A simple and rapid method was investigated for the determination of μg/l levels of lead and cadmium in biological samples, by multichannel graphite furnace atomic absorption spectrometry using ammonium dihydrogen phosphate as a matrix modifier. Bovine whole blood and human serum were selected as biological samples for application. The method has a detection limit of 0.36 μg/l for lead and 0.026 μg/l for cadmium. Concentration of lead 788±14 μg/l and cadmium 13.4±0.1 μg/l in a certified reference material, bovine blood, was in good agreement with the certified value (lead 772±11 μg/l ; cadmium 12.4±0.5 μg/l), respectively. This method was applied to ten sera of normal subject and levels of lead (3.3±1.0 μg/l) and cadmium (0.28±0.11 μg/l) obtained showed reasonable values.