Dynamic interplay between sortilin and syndecan-1 contributes to prostate cancer progression

• Published in: Scientific reports Vol. 13; no. 1; p. 13489
• Main Authors: Lazniewska, Joanna, Li, Ka Lok, Johnson, Ian R. D., Sorvina, Alexandra, Logan, Jessica M., Martini, Carmela, Moore, Courtney, Ung, Ben S.-Y., Karageorgos, Litsa, Hickey, Shane M., Prabhakaran, Sarita, Heatlie, Jessica K., Brooks, Robert D., Huzzell, Chelsea, Warnock, Nicholas I., Ward, Mark P., Mohammed, Bashir, Tewari, Prerna, Martin, Cara, O’Toole, Sharon, Edgerton, Laura Bogue, Bates, Mark, Moretti, Paul, Pitson, Stuart M., Selemidis, Stavros, Butler, Lisa M., O’Leary, John J., Brooks, Douglas A.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.08.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 631/67/1857; 631/67/2327; 631/67/589/466; 631/80/313/1776; Androgen receptors; Androgens; Castration; Glucose; Glucose metabolism; Humanities and Social Sciences; Immunohistochemistry; Lipid metabolism; Lipids; Lipoprotein lipase; Metabolism; Molecular modelling; multidisciplinary; Phenotypes; Prostate cancer; Science; Science (multidisciplinary); Syndecan
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-40347-7

Prostate cancer (PCa) development and progression relies on the programming of glucose and lipid metabolism, and this involves alterations in androgen receptor expression and signalling. Defining the molecular mechanism that underpins this metabolic programming will have direct significance for patients with PCa who have a poor prognosis. Here we show that there is a dynamic balance between sortilin and syndecan-1, that reports on different metabolic phenotypes. Using tissue microarrays, we demonstrated by immunohistochemistry that sortilin was highly expressed in low-grade cancer, while syndecan-1 was upregulated in high-grade disease. Mechanistic studies in prostate cell lines revealed that in androgen-sensitive LNCaP cells, sortilin enhanced glucose metabolism by regulating GLUT1 and GLUT4, while binding progranulin and lipoprotein lipase (LPL) to limit lipid metabolism. In contrast, in androgen-insensitive PC3 cells, syndecan-1 was upregulated, interacted with LPL and colocalised with β 3 integrin to promote lipid metabolism. In addition, androgen-deprived LNCaP cells had decreased expression of sortilin and reduced glucose-metabolism, but increased syndecan-1 expression, facilitating interactions with LPL and possibly β 3 integrin. We report a hitherto unappreciated molecular mechanism for PCa, which may have significance for disease progression and how androgen-deprivation therapy might promote castration-resistant PCa.