Phage display for target-based antibacterial drug discovery

Increasing bacterial drug resistance and hard-to-eradicate opportunistic infections have created a need for new antibiotics. Sequencing of microbial genomes has yielded many new potential targets for antibacterial drug discovery. However, little is known about the biochemical activities of many of t...

Full description

Saved in:
Bibliographic Details
Published inDrug Discovery Today Vol. 6; no. 14; pp. 721 - 727
Main Authors Christensen, Dale J, Gottlin, Elizabeth B, Benson, R.Edward, Hamilton, Paul T
Format Book Review Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 01.07.2001
Elsevier Science
Subjects
Antibacterial agents
antibacterial drugs
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
General pharmacology
high throughput screening
Medical sciences
phage display
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
surrogate ligand
target validation
Techniques & Methods
phage display
target validation
Pharmaceutical Science
high throughput screening
surrogate ligand
antibacterial drugs
Biochemistry
Drug Discovery
Target
Phage display
Peptides
Bacteria
Review
Antibacterial agent
Genome
Pharmaceutical industry
Research and development
Online AccessGet full text

Cover

More Information
Summary:Increasing bacterial drug resistance and hard-to-eradicate opportunistic infections have created a need for new antibiotics. Sequencing of microbial genomes has yielded many new potential targets for antibacterial drug discovery. However, little is known about the biochemical activities of many of these targets, making it difficult to develop HTS assays for them. Peptides isolated by phage display can be used as ‘surrogate ligands’ in competition assays for screening of targets of unknown function with small-molecule libraries. These screening assays can be adapted into a variety of high-throughput formats, including those based on radioactive, luminescence or fluorescence detection.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
ObjectType-Review-3
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:1359-6446
1878-5832
DOI:10.1016/S1359-6446(01)01853-0