The effect of lead on bone mineral properties from female adult C57/BL6 mice

• Published in: Bone (New York, N.Y.) Vol. 47; no. 5; pp. 888 - 894
• Main Authors: Monir, A.U, Gundberg, C.M, Yagerman, S.E, van der Meulen, M.C.H, Budell, W.C, Boskey, A.L, Dowd, T.L
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier 01.11.2010
• Subjects: Animals; Biological and medical sciences; Biomechanical Phenomena - physiology; Blood; Bone Density - drug effects; Calcification, Physiologic - drug effects; Female; Fundamental and applied biological sciences. Psychology; Lead - toxicity; Mice; Mice, Inbred C57BL; Organometallic Compounds - toxicity; Orthopedics; Osteocalcin - metabolism; Osteoporosis - chemically induced; Spectroscopy, Fourier Transform Infrared; Vertebrates: anatomy and physiology, studies on body, several organs or systems; X-Ray Microtomography; Lead; Biomarkers; Mice; Bone; Microcomputed tomography; Fourier Transform Infrared Imaging; Human; Radiodiagnosis; Rodentia; Biological marker; Vertebrata; Mammalia; Mouse; Animal; Morphology; Tomography; Adult; Female
• ISSN: 8756-3282; 1873-2763
• DOI: 10.1016/j.bone.2010.07.013

Abstract Lead toxicity is a significant problem in the U.S. with elevated blood lead levels being highest among very young children and older adults > 50 years old. Bone is the major reservoir of body lead, accounting for 75% in children and 90% in adults. Very little is known about the effect of lead on bone mineral properties in adults. We investigated the effect of lead on the femora from adult, 6 month old female C57/BL6 mice who were administered lead in the drinking water (250 ppm, blood lead 33 μg/dL) for 4 months. Bone mineral properties were examined using Fourier Transform Infrared Microscopy (FTIRM), quantitative microcomputed tomography (microCT) and whole bone mechanical testing. Lead significantly decreased the bone mineral density in the cortical and proximal cancellous bone and increased the marrow area in the cortical bone with microCT. Whole bone three-point bending showed a trend of decreased maximum and failure moments in the lead treated bones compared to controls. Lead significantly decreased the mineral/matrix ratio, collagen maturity and crystallinity in the trabecular bone as measured by FTIRM. In the cortical bone lead significantly decreased collagen maturity and bone crystal size by FTIRM. In contrast to cell culture studies, lead significantly increased serum osteocalcin levels. Lead also significantly increased the bone formation and resorption markers suggesting increased bone turnover. These data show that lead increases bone turnover resulting in weaker cortical bone in adult female mice and suggest that lead may exacerbate bone loss and osteoporosis in the elderly.