IBA and melatonin increase trigonelline and caffeine during the induction and initiation of adventitious roots in Coffea arabica L. cuttings

• Published in: Scientific reports Vol. 13; no. 1; pp. 15151 - 14
• Main Authors: de Andrade, Francisco Hélio Alves, Ferreira, Ana Maria Oliveira, Azevedo, Lillian Magalhães, de Oliveira Santos, Meline, Carvalho, Gladyston Rodrigues, de Resende, Mário Lucio Vilela, Bicalho, Elisa Monteze, Silva, Vânia Aparecida
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 13.09.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 631/449; 631/45; 631/61; Antioxidants; Caffeine; Coffea arabica; Coffee; Cuttings; Developmental stages; Enzymes; Humanities and Social Sciences; Hydrogen peroxide; Indole-3-butyric acid; Melatonin; Metabolites; multidisciplinary; Phenols; Rooting; Roots; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-41288-x

Caffeine and trigonelline are found in Coffea arabica , and show antioxidant roles and growth and development functions. However, there are no reports on trigonelline and caffeine in relation to coffee rooting. The aim was to evaluate the impact of application of indole-3-butyric acid (IBA) and melatonin on caffeine and trigonelline at different stages of adventitious rooting in cuttings. In addition, to study the correlation between these metabolites and H 2 O 2 , phenols, and antioxidant enzymes. Four treatments (Control, melatonin 21 µM (M21), melatonin 43 µM (M43), and IBA 7380 µM (IBA)) were used, with four replications. The growth and biochemical parameters of the antioxidant system were performed in induction, initiation, and extension rooting stages. Higher concentrations of trigonelline and caffeine quantified in the induction and initiation stages were positively correlated with higher percentage of rooted cuttings. Trigonelline and caffeine were positively correlated with H 2 O 2 in all stages of development of adventitious roots. The correlations of trigoneline and caffeine with phenols and antioxidant enzymes reveal different profiles, depending on the phases. The results indicate that IBA and melatonin increase trigonelline and caffeine during the induction and initiation of adventitious roots in Coffea arabica cuttings, which is correlated with a higher percentage of rooted cuttings.