The molecular biology of secreted enzyme production by fungi

• Published in: Critical reviews in biotechnology Vol. 17; no. 4; pp. 273 - 306
• Main Authors: Archer, D.B, Peberdy, J.F
• Format: Journal Article
• Language: English
• Published: Boca Raton, FL Informa UK Ltd 1997; Taylor & Francis; CRC Press
• Subjects: Aspergillus; Aspergillus - genetics; Aspergillus - metabolism; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; CHAMPIGNON; ENZIMAS; ENZYME; ENZYMES; Enzymes - genetics; Enzymes - metabolism; Enzymes - secretion; Fundamental and applied biological sciences. Psychology; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungal Proteins - secretion; FUNGI; fungus; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Fungal; Genetics; glycosylation; heterologous gene expression; HONGOS; Mission oriented research; protein secretion; Trichoderma; Trichoderma - genetics; Trichoderma - metabolism; Trichoderma; Transcription; Enzyme; Secretion; Biosynthesis; Glycosylation; Review; Gene expression; Posttranscriptional modification; Fungi; Aspergillus; Regulation(control); Fungi Imperfecti; Recombinant protein; Thallophyta
• ISSN: 0738-8551; 1549-7801
• DOI: 10.3109/07388559709146616

Abstract Enzymes from filamentous fungi are already widely exploited, but new applications for known enzymes and new enzymic activities continue to be found. In addition, enzymes from less amenable non-fungal sources require heterologous production and fungi are being used as the production hosts. In each case there is a need to improve production and to ensure quality of product. While conventional, mutagenesis-based, strain improvement methods will continue to be applied to enzyme production from filamentous fungi the application of recombinant DNA techniques is beginning to reveal important information on the molecular basis of fungal enzyme production and this knowledge is now being applied both in the laboratory and commercially. We review the current state of knowledge on the molecular basis of enzyme production by filamentous fungi. We focus on transcriptional and post-transcriptional regulation of protein production, the transit of proteins through the secretory pathway and the structure of the proteins produced including glycosylation.