Increase in the amount of celA1 protein in tobacco [Nicotiana tabacum] BY-2 cells by a cellulose biosynthesis inhibitor, 2,6-dichlorobenzonitrile

• Published in: Plant and cell physiology Vol. 39; no. 7; pp. 779 - 785
• Main Authors: Nakagawa, N. (Hiroshima Univ. (Japan). Faculty of Integrated Arts and Sciences), Sakurai, N
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.07.1998
• Subjects: 6-Dichlorobenzonitrile; acclimation; ACCROISSEMENT DE PRODUCTION; Agronomy. Soil science and plant productions; Amino Acid Sequence; amino acid sequences; Anti-peptide antibody; AUMENTO DE PRODUCCION; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; Blotting, Western; carbohydrate metabolism; celAl gene; Cell biochemistry; Cell Line; Cell physiology; cell suspension culture; Cell Wall - metabolism; CELLS; Cellulase - chemistry; Cellulase - metabolism; CELLULE; Cellulose; Cellulose - antagonists & inhibitors; Cellulose - biosynthesis; cellulose synthase; CELLULOSES; CELULAS; Chemical constitution; Economic plant physiology; enzyme inhibitors; ESTIMULO; Fundamental and applied biological sciences. Psychology; hexosyltransferases; immunocytochemistry; INHIBICION; INHIBITION; microsomes; Molecular Sequence Data; Nicotiana - drug effects; Nicotiana - metabolism; NICOTIANA TABACUM; Nicotiana tabacum BY-2; Nitriles - pharmacology; Plant physiology and development; plant proteins; Plants, Toxic; polysaccharides; PRODUCTION INCREASE; PROTEINAS; PROTEINE; PROTEINS; RSW1; Sequence Homology, Amino Acid; STIMULI; STIMULUS; Cell culture; Enzyme; Antibody; Transferases; Glycosyltransferases; Cellulose; Stimulant plant; Biosynthesis; Nicotiana tabacum; Protein; Cellulose synthase (UDP-forming); Dicotyledones; Angiospermae; Hexosyltransferases; Inhibitor; Spermatophyta; Solanaceae; Inhibition; Biological accumulation
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/oxfordjournals.pcp.a029434

The biochemical analysis of cellulose biosynthesis by plants has been a difficult problem due to the lack of a reliable assay procedure for cellulose synthase activity. Recently, the celA1 gene was cloned from cotton fiber, and this gene was identified from the rsw1 mutant of Arabidopsis as a catalytic subunit of cellulose synthase. The cloning of these genes enables us to obtain specific antibodies against cellulose synthase. A highly specific antibody against celA1 protein was prepared and used to detect the protein from microsomal fraction of tobacco BY-2 cells. The quantity of celA1 protein in microsomal fraction of normal BY-2 cells was under the detection limit, although they contained a large quantity of cellulose. In contrast, cells habituated to 1 mu-M DCB (a specific inhibitor of cellulose biosynthesis) produced 1/10 of cellulose content of the normal cells, but had much more celA1 protein than the normal cells. The amount of polysaccharides in the EDTA-soluble fraction was relatively increased in habituated cells. The results suggest that celA1 protein is stabilized upon DCB binding and that the crystallization of cellulose microfibrils is inhibited simultaneously