A Role for the Proteasome Alpha2 Subunit N-Tail in Substrate Processing

• Published in: Biomolecules (Basel, Switzerland) Vol. 13; no. 3; p. 480
• Main Authors: Sahu, Indrajit, Bajorek, Monika, Tan, Xiaolin, Srividya, Madabhushi, Krutauz, Daria, Reis, Noa, Osmulski, Pawel A, Gaczynska, Maria E, Glickman, Michael H
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05.03.2023; MDPI
• Subjects: 20S gating; Biological research; Biology, Experimental; Channel gating; Cloning; Core particles; Genes; Microscopy; Models, Molecular; Mutation; Physiological aspects; Plasmids; proteasome; Proteasome 26S; Proteasome Endopeptidase Complex - metabolism; Proteasomes; Proteins; Proteolysis; Silicon nitride; substrate translocation; Substrates (Biochemistry); ubiquitin; Ubiquitin-proteasome system; United States; proteasome; 20S gating; proteolysis; substrate translocation; ubiquitin
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom13030480

The proteolytic active sites of the 26S proteasome are sequestered within the catalytic chamber of its 20S core particle (CP). Access to this chamber is through a narrow channel defined by the seven outer α subunits. In the resting state, the N-termini of neighboring α subunits form a gate blocking access to the channel. The attachment of the activators or regulatory particles rearranges the blocking α subunit N-termini facilitating the entry of substrates. By truncating or mutating each of the participating α N-termini, we report that whereas only a few N-termini are important for maintaining the closed gate, all seven N-termini participate in the open gate. Specifically, the open state is stabilized by a hydrogen bond between an invariant tyrosine (Y) in each subunit with a conserved aspartate (D) in its counterclockwise neighbor. The lone exception is the α1-α2 pair leaving a gap in the ring circumference. The third residue (X) of this YD(X) motif aligns with the open channel. Phenylalanine at this position in the α2 subunit comes in direct contact with the translocating substrate. Consequently, deletion of the α2 N-terminal tail attenuates proteolysis despite the appearance of an open gate state. In summary, the interlacing N-terminal YD(X) motifs regulate both the gating and translocation of the substrate.