Protein Kinase C Inhibitors Prevent Induction and Continued Expression of Cell Memory in Hermissenda Type B Photoreceptors

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 88; no. 5; pp. 2016 - 2020
• Main Authors: Farley, Joseph, Schuman, Erin
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.03.1991; National Acad Sciences
• Subjects: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; Alkaloids - pharmacology; Animals; B lymphocytes; Biochemistry. Physiology. Immunology; Biological and medical sciences; Calcium - pharmacology; Cells; Depolarization; Electric current; Electric Stimulation; enzymatic activity; enzyme inhibitors; Enzymes; Fundamental and applied biological sciences. Psychology; Hermissenda crassicornis; In Vitro Techniques; Invertebrates; Isoquinolines - pharmacology; Marine; Membrane potential; Memory; Microelectrodes; Mollusca; Nervous system; Nervous System Physiological Phenomena; Photoreceptor Cells - drug effects; Photoreceptor Cells - physiology; Photoreceptors; Physiology. Development; Piperazines - pharmacology; protein kinase C; Protein Kinase C - antagonists & inhibitors; sense organs; Snails - physiology; Space life sciences; Sphingosine - pharmacology; Staurosporine; Steady state current; Protein kinase C; Calcium; Photoreceptor; Electrophysiology; Excitability; Enzyme inhibitor; Nervous system; Gastropoda; Ionic current; Sensory receptor; Marine environment; Hermissenda crassicornis; Invertebrata; Mollusca; Potassium
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.88.5.2016

Injections of cAMP-dependent, Ca2+/calmodulin-dependent, Ca2+/phospholipid-dependent protein kinases into Hermissenda crassicornis type B photoreceptors are sufficient to induce many of the changes in B-cell excitability produced by associative conditioning. We report that inhibitors of Ca2+/phospholipid-dependent protein kinases, but not inhibitors of cyclic nucleotide- or Ca2+/calmodulin-dependent protein kinases, prevent the induction as well as continued expression of learning-produced changes in type-B-cell excitability: reductions of voltage-dependent and Ca2+-activated K+currents. Our results represent a direct demonstration of long-term (days) experientially induced modulation of ion-channel activity that is dependent upon persistent kinase activity.