Plasminogen Activator Inhibitor-1 4G/5G (rs1799889) Polymorphism in Chinese Patients with Diabetes Mellitus and Hypertension

• Published in: Diabetes, metabolic syndrome and obesity Vol. 16; pp. 1133 - 1147
• Main Authors: Wang, Ziran, Chen, Jintu, Song, Jingjing, Kong, Lingjun, Wang, Xuanzhi, Xu, Tengda, Lin, Songbai, Dou, Yaling
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2023; Dove; Dove Medical Press
• Subjects: Analysis; Diabetes; diabetes mellitus; Diabetics; Disease susceptibility; gene polymorphism; Genes; Genetic aspects; Genetic polymorphisms; Health aspects; Hypertension; Medical research; Medicine, Experimental; Original Research; plasminogen activator inhibitor-1; China; gene polymorphism; diabetes mellitus; plasminogen activator inhibitor-1; hypertension
• ISSN: 1178-7007; 1178-7007
• DOI: 10.2147/DMSO.S410682

To determine the plasminogen activator inhibitor-1 (PAI-1) 4G/5G (rs1799889) genotype of the subjects in a robust detection method and to explore the association of the 4G/5G polymorphism with susceptibility to diabetes mellitus (DM) and hypertension (HTN) as well as clinical characteristics. This study recruited 208 patients (68 patients were diagnosed with DM, 70 patients with HTN and 70 patients with DM combined with HTN) and 132 healthy controls (HC). A subset of the population was selected to evaluate the accuracy of the Real-time PCR (RT-PCR) method for detecting 4G/5G polymorphism by using the sequencing method as the gold standard. Furthermore, the association of the 4G/5G polymorphism with genetic susceptibility to DM and HTN was explored. Moreover, variations in clinical characteristics among individuals with various genotypes were also analyzed in the DM group, the HTN group and the DM+HTN group. There was a high concordance between the RT-PCR method and the sequencing method in determining the 4G/5G polymorphism. No association was observed between the 4G/5G polymorphism and susceptibility to DM, HTN and DM+HTN, respectively. There were no statistical differences in all study indicators among individuals that carrying various genotypes in the HC group. There were several variations in clinical characteristics among individuals harboring different 4G/5G genotypes in the DM group, the HTN group and the DM+HTN group. The RT-PCR method can accurately identify the 4G/5G genotype in different individuals. The 4G/5G polymorphism may not be associated with genetic susceptibility to DM, HTN and DM+HTN, but differences in clinical characteristics among individuals with various genotypes may provide a reference for disease assessment and personalized treatment of patients.