Strain-specific differences in cell proliferation and differentiation in the dentate gyrus of C57BL/6N and C3H/HeN mice fed a high fat diet

• Published in: Brain research Vol. 1241; pp. 1 - 6
• Main Authors: Hwang, In Koo, Kim, Il Yong, Kim, Dae Won, Yoo, Ki-Yeon, Kim, Yo Na, Yi, Sun Shin, Won, Moo-Ho, Lee, In Se, Yoon, Yeo Sung, Seong, Je Kyung
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 19.11.2008
• Subjects: Animals; Biomarkers - analysis; Biomarkers - metabolism; Body Weight - genetics; C3H/HeN; C57BL/6N; Cell Differentiation - genetics; Cell Proliferation; Dentate gyrus; Dentate Gyrus - cytology; Dentate Gyrus - drug effects; Dietary Fats - toxicity; Disease Models, Animal; Doublecortin; Food, Formulated - toxicity; Genetic Predisposition to Disease; High fat diet; Immunohistochemistry; Ki-67; Ki-67 Antigen - analysis; Ki-67 Antigen - metabolism; Male; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Microtubule-Associated Proteins - analysis; Microtubule-Associated Proteins - metabolism; Neurology; Neuronal Plasticity - genetics; Neurons - cytology; Neurons - metabolism; Neuropeptides - analysis; Neuropeptides - metabolism; Obesity; Obesity - genetics; Obesity - metabolism; Species Specificity; Stem Cells - cytology; Stem Cells - metabolism; Weight Gain - genetics; C57BL/6N; Ki-67; Obesity; Dentate gyrus; Doublecortin; High fat diet; C3H/HeN
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2008.08.024

Abstract The authors investigated strain-specific cell proliferation and differentiation differences in the dentate gyri of C57BL/6N (susceptible strain to obesity) and C3H/HeN (resistant strain to obesity) mice. In addition, the influences of a high fat diet (HD) on neuronal differentiation in C57BL/6N and C3H/HeN mice fed a low-fat diet (LD) or HD for 4 or 12 weeks were investigated. Body weight and body weight gains were significantly higher in HD-fed C57BL/6N and C3H/HeN mice than in LD-fed C57BL/6N and C3H/HeN mice. In particular, body weight gains were significantly higher in C57BL/6N mice than in C3H/HeN mice. In both of HD- and LD-fed C57BL/6N and C3H/HeN mice for 4 weeks, some Ki67 and many DCX immunoreactive cells were detected in the subgranular zone of the dentate gyrus. In HD-fed C57BL/6N and C3H/HeN mice, the number of Ki67 immunoreactive cells and DCX immunoreactivities in the dentate gyri were significantly lower than in LD-fed C57BL/6N and C3H/HeN mice. However, the number of Ki67 immunoreactive cells and DCX immunoreactivities in HD-fed C57BL/6N mice were significantly lower than in HD-fed C3H/HeN mice. These results suggest that C57BL/6N mice are more vulnerable to HD induced obesity than C3H/HeN mice. In addition, the feeding of HD was found to exacerbate reduced cell proliferation and differentiation in the dentate gyri of C57BL/6N mice as compared with that in C3H/HeN mice.