Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection

• Published in: Genomics (San Diego, Calif.) Vol. 91; no. 5; pp. 423 - 432
• Main Authors: Mukherjee, Shankar, Nagajyothi, Fnu, Mukhopadhyay, Aparna, Machado, Fabiana S., Belbin, Thomas J., Campos de Carvalho, Antonio, Guan, Fangxia, Albanese, Chris, Jelicks, Linda A., Lisanti, Michael P., Silva, Joao S., Spray, David C., Weiss, Louis M., Tanowitz, Herbert B.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.05.2008; Elsevier
• Subjects: Animals; Biological and medical sciences; Cardiomyopathy; Chagas Cardiomyopathy - genetics; Chagas Cardiomyopathy - microbiology; Chagas Cardiomyopathy - pathology; Chagas disease; Cluster Analysis; Fas; Fundamental and applied biological sciences. Psychology; Gene array analysis; Gene Expression Profiling; Gene Expression Regulation; Genes. Genome; Genetics of eukaryotes. Biological and molecular evolution; Human protozoal diseases; Infection/inflammation; Infectious diseases; Male; Medical sciences; Mice; Models, Animal; Molecular and cellular biology; Molecular genetics; Myocardium - metabolism; Oligonucleotide Array Sequence Analysis; Parasitic diseases; Protozoal diseases; Trypanosoma cruzi; Trypanosoma cruzi - physiology; Trypanosoma cruzi; Cyclins; Trypanosomiasis; Chagas disease; Fas; Trypanosoma cruzi; Cyclins; Infection/inflammation; Cardiomyopathy; Gene array analysis; Kinetoplastida; Protozoa; Protozoal disease; Genomics; Alteration; Cyclin; Cardiovascular disease; Inflammation; Parasitosis; Experimental study; Gene expression; Myocardial disease; Cyclins; Trypanosoma cruzi; Infection; Gene; Heart disease; Trypanosomiasis
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2008.01.008

Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence-verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with dye reversal. We identified eight distinct categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention.