Expression of heat shock protein (Hsp) 70 and Hsp 40 in gastric cancer

• Published in: Cancer letters Vol. 198; no. 2; pp. 219 - 228
• Main Authors: Isomoto, Hajime, Oka, Mikio, Yano, Yoshitsugu, Kanazawa, Yusei, Soda, Hiroshi, Terada, Ryusuke, Yasutake, Toru, Nakayama, Toshiyuki, Shikuwa, Saburo, Takeshima, Fuminao, Udono, Heiichiro, Murata, Ikuo, Ohtsuka, Kenzo, Kohno, Shigeru
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.08.2003; Elsevier; Elsevier Limited
• Subjects: Adult; Aged; Aged, 80 and over; Antineoplastic agents; Autoimmune diseases; Biological and medical sciences; Female; Gastric cancer; Heat shock protein 40; Heat shock protein 70; Heat-Shock Proteins - metabolism; HSP40 Heat-Shock Proteins; HSP70 Heat-Shock Proteins - metabolism; Humans; Immunoblot; Immunoblotting; Immunohistochemistry; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Liver Neoplasms - secondary; Male; Medical sciences; Middle Aged; Multivariate Analysis; Neoplasm Metastasis; Neoplasm Staging; Pharmacology. Drug treatments; Polyclonal antibodies; Proteins; Stomach Neoplasms - pathology; Stomach Neoplasms - surgery; Tumors; Immunohistochemistry; Immunoblot; Heat shock protein 70; Heat shock protein 40; Gastric cancer; Heat shock protein Hsp70; Stomach; Digestive system; Heat shock protein; Immunoblotting assay; Malignant tumor; Gene expression
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/S0304-3835(03)00305-7

Heat shock proteins (Hsp) 70 and Hsp 40 are stress proteins that cooperate as chaperones in mammalian cells. We determined the expression of Hsp 70 and Hsp 40 in 81 gastric cancers. Immunoreactivities to Hsp 70 and Hsp 40 were detected in 67.9 and 22.2% of tumors, respectively. Immunohistochemical analysis showed enhanced Hsp 70 and Hsp 40 expression in gastric tumor tissue, relative to the surrounding normal tissue. Overexpression of Hsp 70 and Hsp 40 was also confirmed by immunoblotting. Among various clinicopathological parameters, low histopathological differentiation was associated with reduced expression of both proteins.