Genome-Wide Identification and Analysis of OsSPXs Revealed Its Genetic Influence on Cold Tolerance of Dongxiang Wild Rice (DXWR)

• Published in: International journal of molecular sciences Vol. 24; no. 10; p. 8755
• Main Authors: Huang, Cheng, Wang, Jilin, Wang, Dianwen, Chang, Jingjing, Chen, Hongping, Chen, Dazhou, Deng, Wei, Tian, Chunjie
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.05.2023; MDPI
• Subjects: Agricultural production; Analysis; Cellular signal transduction; Climate change; Cold; Cold Temperature; Cold tolerance; Cold treatment; Dongxiang wild rice; Evolution; expression patterns; Gene amplification; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes; Genetic analysis; Genomes; Genomics; Information management; Oryza - physiology; Oryza rufipogon Griff; Phosphates; Phylogeny; Plant Breeding; Plant growth; Proteins; Seeds; Signal transduction; SPX-domain protein; Stress, Physiological - genetics; Temperature; Transcriptome; Transcriptomes; Yeast; China; Dongxiang wild rice; Oryza rufipogon Griff; cold tolerance; expression patterns; SPX-domain protein
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24108755

SPX-domain proteins (small proteins with only the SPX domain) have been proven to be involved in phosphate-related signal transduction and regulation pathways. Except for research showing that it plays a role in the process of rice adaptation to cold stress, the potential functions of other genes in cold stress are unknown. Therefore, in this study, we identified six from the whole genome of DXWR. The phylogeny of has a strong correlation with its motif. Transcriptome data analysis showed that were highly sensitive to cold stress, and real-time PCR verified that the levels of , , , and in cold-tolerant materials (DXWR) during cold treatment were higher than that of cold-sensitive rice (GZX49). The promoter region of DXWR contains a large number of cis-acting elements related to abiotic stress tolerance and plant hormone response. At the same time, these genes have expression patterns that are highly similar to cold-tolerance genes. This study provides useful information about , which is helpful for the gene-function research of DXWR and genetic improvements during breeding.