Locally Controlled Diffusive Release of Bone Morphogenetic Protein-2 Using Micropatterned Gelatin Methacrylate Hydrogel Carriers

• Published in: Biochip journal Vol. 14; no. 4; pp. 405 - 420
• Main Authors: Yi, Myong-Hee, Lee, Ji-Eun, Kim, Chang-Beom, Lee, Keun-Woo, Lee, Kwang-Ho
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Applied Biological Chemistry 01.12.2020; Springer Nature B.V; 한국바이오칩학회
• Subjects: Actin; Biomedical Engineering and Bioengineering; Biotechnology; Bone growth; Bone morphogenetic protein 2; Bone morphogenetic proteins; Bovine serum albumin; Cell differentiation; Chemistry; Collagen; Connective tissues; Contact angle; Controlled release; Cytokines; Differentiation (biology); Fluorescein isothiocyanate; Gelatin; Hydrogels; Localization; Membranes; Micropatterning; Nanotechnology; Original; Original Article; Osteosarcoma; Proteins; Regeneration; Regeneration (physiology); Regenerative medicine; Serum albumin; Spectrum analysis; Tissue engineering; 생물공학; Bone morphogenetic protein-2; Bone generation; Gelatin-methacrylate; Controlled release; Localized release
• ISSN: 1976-0280; 2092-7843
• DOI: 10.1007/s13206-020-4411-0

In this work, a novel and simple bone morphogenetic protein (BMP)-2 carrier is developed, which enables localized and controlled release of BMP-2 and facilitates bone regeneration. BMP-2 is localized in the gelatin methacrylate (GelMA) micropatterns on hydrophilic semi-permeable membrane (SNM), and its controlled release is regulated by the concentration of GelMA hydrogel and BMP-2. The controlled release of BMP-2 is verified using computational analysis and quantified using fluorescein isothiocyanate-bovine serum albumin (FITC-BSA) diffusion model. The osteogenic differentiation of osteosarcoma MG-63 cells is manipulated by localized and controlled BMP-2 release. The calcium deposits are significantly higher and the actin skeletal networks are denser in MG-63 cells cultured in the BMP-2-immobilized GelMA micropattern than in the absence of BMP-2. The proposed BMP-2 carrier is expected to not only act as a barrier membrane that can prevent invasion of connective tissue during bone regeneration, but also as a carrier capable of localizing and controlling the release of BMP-2 due to GelMA micropatterning on SNM. This approach can be extensively applied to tissue engineering, including the localization and encapsulation of cells or drugs.