An Isolated Arthrobacter sp. Enhances Rice (Oryza sativa L.) Plant Growth

• Published in: Microorganisms (Basel) Vol. 10; no. 6; p. 1187
• Main Authors: Chhetri, Geeta, Kim, Inhyup, Kang, Minchung, So, Yoonseop, Kim, Jiyoun, Seo, Taegun
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 09.06.2022; MDPI
• Subjects: anti-infective agents; Antiinfectives and antibacterials; Antimicrobial agents; Arthrobacter; Bacteria; biofilm; Biofilms; Bioremediation; Climate change; culture-dependent; Genomes; greenhouses; Hormones; humans; IAA; Inoculation; L-tryptophan; Metabolites; Nitrogen; Oryza sativa; paddies; Pathogens; PGP; Plant growth; Plant growth promoters; Rhizosphere; rhizosphere bacteria; Rice; Rice fields; Roots; Scanning electron microscopy; Secondary metabolites; siderophore; South Korea; United States > US; South Korea
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms10061187

Rice is a symbol of life and a representation of prosperity in South Korea. However, studies on the diversity of the bacterial communities in the rhizosphere of rice plants are limited. In this study, four bundles of root samples were collected from the same rice field located in Goyang, South Korea. These were systematically analyzed to discover the diversity of culturable bacterial communities through culture-dependent methods. A total of 504 culturable bacteria were isolated and evaluated for their plant growth-promoting abilities in vitro. Among them, Arthrobacter sp. GN70 was selected for inoculation into the rice plants under laboratory and greenhouse conditions. The results showed a significantly positive effect on shoot length, root length, fresh plant weight, and dry plant weight. Moreover, scanning electron microscopic (SEM) images demonstrated the accumulation of bacterial biofilm networks at the junction of the primary roots, confirming the root-colonizing ability of the bacterium. The strain also exhibited a broad spectrum of in vitro antimicrobial activities against bacteria and fungi. Here, we first report the rice plant growth-promoting ability of the Arthrobacter species with the biofilm-producing and antimicrobial activities against plant and human pathogens. Genome analyses revealed features attributable to enhance rice plant growth, including the genes involved in the synthesis of plant hormones, biofilm production, and secondary metabolites. This study revealed that the rhizobacteria isolated from the roots of rice plants have dual potential to be utilized as a plant growth promoter and antimicrobial agent.