Antioxidant and immunological activity in vitro of polysaccharides from Gomphidius rutilus mycelium

• Published in: Carbohydrate polymers Vol. 92; no. 2; pp. 2187 - 2192
• Main Authors: Gao, Chanjuan, Wang, Yanhua, Wang, Chenyu, Wang, Zhanyong
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15.02.2013; Elsevier
• Subjects: 2,2-diphenyl-1-picrylhydrazyl; Adjuvants, Immunologic - chemistry; Adjuvants, Immunologic - isolation & purification; Adjuvants, Immunologic - pharmacology; Animals; Antioxidant; Antioxidants; Applied sciences; Basidiomycota - chemistry; Biological and medical sciences; Carbohydrates; Cell Proliferation - drug effects; Concanavalin A; Exact sciences and technology; Fermentation; Free Radical Scavengers - chemistry; Free Radical Scavengers - isolation & purification; Free Radical Scavengers - pharmacology; Free radicals; General and cellular metabolism. Vitamins; Glucose; Gomphidius; Gomphidius Rutilus; hydroxyl radicals; Immunomodulators; Lipopolysaccharides; lymphocyte proliferation; Lymphocytes; Lymphocytes - cytology; Lymphocytes - drug effects; Male; Medical sciences; Mice; Mitogens; Molecular weight; Mycelium; Mycelium - chemistry; Natural polymers; Pharmacology. Drug treatments; Physicochemistry of polymers; Polysaccharide; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Purification; Starch and polysaccharides; submerged fermentation; Superoxide; superoxide anion; Xylose; Purification; Antioxidant; Mycelium; Polysaccharide; Gomphidius Rutilus; Cell proliferation; Molecular structure; Chemical isolation; Splenocyte; Solvent extraction; Basidiomycota; Medicinal plant; Fungi; Radical trapping; Chinese medicine; Scavenging efficiency; Folk medicine; Hydroxyl radicals; Edible fungi; DPPH; Experimental study; In vitro; Biological activity; Immunostimulant agent; Plant origin; Oside polymer; Lymphocyte
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2012.12.011

► Two novel polysaccharide fractions were isolated from Gomphidius rutilus mycelium. ► GRMP1 possessed better antioxidant activity in vitro than GRMP2. ► GRMP1 and GRMP2 have significant lymphocyte proliferation activity. Two novel polysaccharide fractions, GRMP1 and GRMP2, were isolated from the mycelium of Gomphidius rutilus through submerged fermentation. GRMP1 and GRMP2 had similar average molecular weights (35 and 31kDa, respectively), and were composed of glucose and xylose at molar ratios of 0.46:1 and 0.63:1, respectively. In vitro antioxidant tests showed that GRMP1 and GRMP2 partly scavenged superoxide radical but almost had no scavenging effect on 1,1-diphenyl-2-picrylhydrazyl free radical. The effect of GRMP1 on hydroxyl radicals was stronger than that of GRMP2. Both GRMP1 and GRMP2 had relatively low reducing power and significant lymphocyte proliferation activity. In the presence of concanavalin A or lipopolysaccharide as mitogens for lymphocytes, the lymphocyte proliferation activity increased for GRMP1 but not for GRMP2 within the test dosage range.