Genome-Wide Identification and Analysis of Stress Response of Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase Genes in Quinoa

• Published in: International journal of molecular sciences Vol. 24; no. 8; p. 6950
• Main Authors: Wang, Xiaoting, Wang, Mingyu, Huang, Yongshun, Zhu, Peng, Qian, Guangtao, Zhang, Yiming, Liu, Yuqi, Zhou, Jingwen, Li, Lixin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.04.2023; MDPI
• Subjects: Amino acids; Analysis; Chenopodium quinoa - genetics; Chenopodium quinoa - metabolism; CqTPS and CqTPP gene families; Enzymes; Food supply; Gene Expression Regulation, Plant; Genomics; Glucosyltransferases - genetics; Glucosyltransferases - metabolism; Metabolites; Phosphatases; Phosphates; Phylogeny; Physiological aspects; Quinoa; saline-alkali stress; Sustainable development; transcriptomics and metabolomics; Trehalose - genetics; Trehalose - metabolism; trehalose biosynthesis; CqTPS and CqTPP gene families; trehalose biosynthesis; transcriptomics and metabolomics; saline-alkali stress; quinoa
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24086950

Saline-alkali stress seriously affects the yield and quality of crops, threatening food security and ecological security. Improving saline-alkali land and increasing effective cultivated land are conducive to sustainable agricultural development. Trehalose, a nonreducing disaccharide, is closely related to plant growth and development and stress response. Trehalose 6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) are key enzymes catalyzing trehalose biosynthesis. To elucidate the effects of long-term saline-alkali stress on trehalose synthesis and metabolism, we conducted an integrated transcriptome and metabolome analysis. As a result, 13 and 11 genes were identified in quinoa ( Willd.) and were named and according to the order of their Gene IDs. Through phylogenetic analysis, the CqTPS family is divided into two classes, and the CqTPP family is divided into three classes. Analyses of physicochemical properties, gene structures, conservative domains and motifs in the proteins, and cis-regulatory elements, as well as evolutionary relationships, indicate that the TPS and TPP family characteristics are highly conserved in quinoa. Transcriptome and metabolome analyses of the sucrose and starch metabolism pathway in leaves undergoing saline-alkali stress indicate that and Class II genes are involved in the stress response. Moreover, the accumulation of some metabolites and the expression of many regulatory genes in the trehalose biosynthesis pathway changed significantly, suggesting the metabolic process is important for the saline-alkali stress response in quinoa.