Acute and chronic effects of exposure to a 1-mT magnetic field on the cytoskeleton, stress proteins, and proliferation of astroglial cells in culture

• Published in: Environmental research Vol. 98; no. 3; pp. 355 - 362
• Main Authors: Bodega, G., Forcada, I., Suárez, I., Fernández, B.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.07.2005; Elsevier
• Subjects: Actin; Actins - metabolism; Animals; Astrocyte; Astrocytes - cytology; Astrocytes - metabolism; Astrocytes - radiation effects; Biological and medical sciences; Cell Proliferation - radiation effects; Cells, Cultured; Cytoskeleton - metabolism; Cytoskeleton - radiation effects; Effects of various physical factors on living matter (vibrations, electric field, ultrasound, sound...); Electromagnetic Fields - adverse effects; Fundamental and applied biological sciences. Psychology; GFAP; Heat shock proteins; Heat-Shock Proteins - metabolism; Humans; Magnetic field; Tissues, organs and organisms biophysics; Toxicity Tests, Acute; Toxicity Tests, Chronic; Heat shock proteins; Astrocyte; Actin; Magnetic field; GFAP; Cell proliferation; Toxicity; Neuroglia; Acute; In vitro; Stress; Chronic; Heat shock protein; Cytoskeleton; Biological effect
• ISSN: 0013-9351; 1096-0953
• DOI: 10.1016/j.envres.2004.12.010

This paper reports the effects of exposure to static, sinusoidal (50 Hz), and combined static/sinusoidal magnetic fields on cultured astroglial cells. Confluent primary cultures of astroglial cells were exposed to a 1-mT sinusoidal, static, or combined magnetic field for 1 h. In another experiment, cells were exposed to the combined magnetic field for 1, 2, and 4 h. The hsp25, hsp60, hsp70, actin, and glial fibrillary acidic protein contents of the astroglial cells were determined by immunoblotting 24 h after exposure. No significant differences were seen between control and exposed cells with respect to their contents of these proteins, neither were any changes in cell morphology observed. In a third experiment to determine the effect of a chronic (11-day) exposure to a combined 1-mT static/sinusoidal magnetic field on the proliferation of cultured astroglial cells, no significant differences were seen between control, sham-exposed, or exposed cells. These results suggest that exposure to 1-mT sinusoidal, static, or combined magnetic fields has no significant effects on the stress, cytoskeletal protein levels in, or proliferation of cultured astroglial cells.