Characterization of 18 new BoLA-DRB3 alleles

The second exon of the bovine MHC class II DRB3 gene was amplified by polymerase chain reaction (PCR) from DNA samples of 568 zebu Brahman cattle (Bos indicus) from Martinique (French West Indies). Cloning of these PCR products allowed the isolation of both alleles from each animal, which were chara...

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Bibliographic Details
Published inAnimal genetics Vol. 30; no. 3; pp. 200 - 203
Main Authors Maillard, J.C, Renard, C, Chardon, P, Chantal, I, Bensaid, A
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.06.1999
Wiley Subscription Services, Inc
Wiley-Blackwell
Subjects
Alleles
Amino Acid Sequence
amino acid sequences
amino-acid sequences BoLA-DRB3 exon 2
Animals
Cattle - genetics
Cattle - immunology
Cloning, Molecular
Exons
genbank/aj001999
genbank/aj002000
genbank/aj002001
genbank/aj002002
genbank/aj002003
genbank/aj002004
genbank/z82023
genbank/z82024
genbank/z82025
genbank/z82027
genbank/z82028
genbank/z82029
genbank/z82030
genbank/z82031
genbank/z82032
genbank/z82033
genbank/z82034
genbank/z82035
Genes, MHC Class II
genetic polymorphism
genetic techniques and protocols
Genetics
Histocompatibility Antigens Class II - genetics
Life Sciences
major histocompatibility complex
Molecular Sequence Data
nucleotide sequences
PCR-RFLP
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
restriction fragment length polymorphism
Sequence Homology, Amino Acid
Terminology as Topic
zebu
zebu Brahman cattle
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Summary:The second exon of the bovine MHC class II DRB3 gene was amplified by polymerase chain reaction (PCR) from DNA samples of 568 zebu Brahman cattle (Bos indicus) from Martinique (French West Indies). Cloning of these PCR products allowed the isolation of both alleles from each animal, which were characterized by the PCR-restriction fragment length polymorphism (RFLP) technique using the restriction enzymes RsaI, BstYI and HaeIII. Four new PGR-RFLP patterns were obtained by digestion with RsaI. These patterns were named 'v', 'w', 'x' and 'y' continuing the accepted nomenclature. Sequencing of each allele allowed the identification of 18 new BoLA-DRB3 exon 2 nucleotide sequences and their deduced amino acid sequences.
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ISSN:0268-9146
1365-2052
DOI:10.1046/j.1365-2052.1999.00446.x