Characterization of 18 new BoLA-DRB3 alleles
The second exon of the bovine MHC class II DRB3 gene was amplified by polymerase chain reaction (PCR) from DNA samples of 568 zebu Brahman cattle (Bos indicus) from Martinique (French West Indies). Cloning of these PCR products allowed the isolation of both alleles from each animal, which were chara...
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Published in | Animal genetics Vol. 30; no. 3; pp. 200 - 203 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.06.1999
Wiley Subscription Services, Inc Wiley-Blackwell |
Subjects | |
Online Access | Get full text |
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Summary: | The second exon of the bovine MHC class II DRB3 gene was amplified by polymerase chain reaction (PCR) from DNA samples of 568 zebu Brahman cattle (Bos indicus) from Martinique (French West Indies). Cloning of these PCR products allowed the isolation of both alleles from each animal, which were characterized by the PCR-restriction fragment length polymorphism (RFLP) technique using the restriction enzymes RsaI, BstYI and HaeIII. Four new PGR-RFLP patterns were obtained by digestion with RsaI. These patterns were named 'v', 'w', 'x' and 'y' continuing the accepted nomenclature. Sequencing of each allele allowed the identification of 18 new BoLA-DRB3 exon 2 nucleotide sequences and their deduced amino acid sequences. |
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Bibliography: | ArticleID:AGE446 ark:/67375/WNG-BK4SBK8W-X istex:6F58CDE46A7D6C2C891F0CE04F8C313A9C103902 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0268-9146 1365-2052 |
DOI: | 10.1046/j.1365-2052.1999.00446.x |