Transcriptional and proteomic profiling of flatfish (Solea senegalensis) spermatogenesis

The Senegalese sole (Solea senegalensis) is a marine flatfish of high economic value and a target species for aquaculture. The efforts to reproduce this species in captivity have been hampered by the fact that farmed males (F1) often show lower sperm production and fertilization capacity than wild‐t...

Full description

Saved in:
Bibliographic Details
Published inProteomics (Weinheim) Vol. 11; no. 11; pp. 2195 - 2211
Main Authors Forné, Ignasi, Castellana, Bárbara, Marín-Juez, Rubén, Cerdà, Joan, Abián, Joaquín, Planas, Josep V.
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 01.06.2011
WILEY‐VCH Verlag
Wiley-VCH
Wiley Subscription Services, Inc
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:The Senegalese sole (Solea senegalensis) is a marine flatfish of high economic value and a target species for aquaculture. The efforts to reproduce this species in captivity have been hampered by the fact that farmed males (F1) often show lower sperm production and fertilization capacity than wild‐type males (F0). Our knowledge on spermatogenesis is however limited to a few studies. In a previous work, we identified by 2‐D DIGE several potential protein markers in testis for the poor reproductive performance of F1 males. Therefore, the objectives of the present study were, first, to investigate changes in genes and proteins expressed in the testis throughout spermatogenesis in F0 males by using a combination of transcriptomic and proteomic approaches and, second, to further compare the testis proteome between late spermatogenic stages of F0 and F1 fish to identify potential indicators of hampered reproductive performance in F1 fish. We identified approximately 400 genes and 49 proteins that are differentially expressed during the progression of spermatogenesis and that participate in processes such as transcriptional activation, the ubiquitin–proteasome system, sperm maturation and motility or cytoskeletal remodeling. Interestingly, a number of these proteins differed in abundance between F0 and F1 fish, pointing toward alterations in cytoskeleton, sperm motility, the ubiquitin–proteasome system and the redox state during spermiogenesis as possible causes for the decreased fertility of F1 fish.
Bibliography:istex:5A9B6A3DB206006CB50DE7FF337EE60B78E2BBD6
Scientific Park of Barcelona (University of Barcelona)
Montserrat Carrascal (CSIC/UAB Proteomics Laboratory)
Colour Online: See article online to view Figs. 1, 2 and 7 in colour.
ArticleID:PMIC201000296
Spanish Ministry of Education and Science (FPI program)
PLEUROGENE project funded by a Genome Spain-Genome Canada joint program
ark:/67375/WNG-FGPH0Q17-9
Oryzon Genomics, Barcelona, Spain
Esther Asensio and Pedro Cañavate (IFAPA, Cádiz)
See article online to view Figs.
1
2
in colour.
and
7
,
Colour Online
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.201000296