Characterization of the quantitatively major collagen in the mantle of oyster Crassostrea gigas

A quantitatively major collagen was isolated from the pepsin-solubilized collagen preparation of the mantle by differential salt precipitation and phosphocellulose column chromatography, and its constituent alpha components (alpha1 and alpha2) were purified by phosphocellulose column chromatography....

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Published inFisheries science Vol. 71; no. 1; pp. 229 - 235
Main Authors Mizuta, S. (Fukui Prefectural Univ., Obama (Japan)), Miyagi, T, Yoshinaka, R
Format Journal Article
LanguageEnglish
Published Melbourne, Australia Blackwell Science Pty 01.02.2005
Springer Nature B.V
Subjects
Amino acids
bivalve
CHEMICOPHYSICAL PROPERTIES
Chromatography
COLLAGEN
CRASSOSTREA GIGAS
ISOLATION
mantle
Margarine
Marine
mollusc
muscle
oyster
Oysters
Peptides
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Summary:A quantitatively major collagen was isolated from the pepsin-solubilized collagen preparation of the mantle by differential salt precipitation and phosphocellulose column chromatography, and its constituent alpha components (alpha1 and alpha2) were purified by phosphocellulose column chromatography. The subunits were demonstrated to be genetically distinct from each other by peptide mapping and amino acid analysis. The amino acid composition calculated from those of the alpha1 and alpha2 components in 2:1 ratio coincided well with that of the major collagen from the mantle. These results suggest that the major collagen in the mantle of the oyster may have a heterotrimer structure (alpha1 )2 alpha2.
Bibliography:2006001439
Q04
L50
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ISSN:0919-9268
1444-2906
DOI:10.1111/j.1444-2906.2005.00952.x