In vitro cellular responses in the RTG-2 cell line to complex mixtures of dioxins and dioxin-like PCDDs, PCDFs and PCBs

• Published in: Journal of applied toxicology Vol. 30; no. 6; pp. 603 - 610
• Main Authors: del Mar Babín, María, Sanz, Paloma, Concejero, Miguel Angel, Martínez, María Angeles, Tarazona, José Vicente
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.08.2010; Wiley
• Subjects: Animals; Benzofurans - analysis; Bioassay; Biological and medical sciences; Biological Assay - methods; Cell Culture Techniques; Cell Line; Complex Mixtures - analysis; Correlation; Correlation coefficients; Cytochrome P-450 CYP1A1 - metabolism; Dioxins; Dioxins - analysis; Dose-Response Relationship, Drug; Enzyme Induction; EROD; Gas Chromatography-Mass Spectrometry; hazardous and toxic waste; Hazardous Waste - analysis; HRGC/MS; In vitro testing; Liquids; Mathematical analysis; Medical sciences; Oncorhynchus mykiss; Polychlorinated Biphenyls - analysis; Polychlorinated Dibenzodioxins - analogs & derivatives; Polychlorinated Dibenzodioxins - analysis; Polymers - analysis; RTG-2; Toxicology; Wastes; RTG-2; EROD; Cell line; Toxicity; Established cell line; Polychlorobiphenyls; hazardous and toxic waste; Hazardous waste; HRGC/MS; Complexes; In vitro; Mixture
• ISSN: 0260-437X; 1099-1263; 1099-1263
• DOI: 10.1002/jat.1532

High‐resolution gas chromatography/mass spectrometry (HRGC/MS) is the standard method for analysing dioxin, furan and polybrominated retardants in hazardous waste. Determination of dioxin‐like compounds using in vitro bioassays such as ethoxyresorufin‐O‐deethylase (EROD) is an important tool to evaluate their Ah receptor‐mediated toxic effects, because it detects all arylhydrocarbon receptor ligands in a variety of sample matrices. In the present work, we compared RTG‐2 cell line EROD bioassay with HRGC/MS for assessing waste samples (liquid and solid) contaminated with polychlorinated dibenzo‐p‐dioxins and dibenzofurans, polychlorinated biphenyls (dioxin‐like PCBs) and other xenobiotics. For liquid samples, HRGC/MS‐toxic equivalent (HRGC/MS‐TEQ) values ranged from 273.26 to 5.84 ng TEQ l−1 and correlated well (correlation coefficient 0.99) with values obtained by EROD‐TEQ, which ranged from 128 to 2.5 ng TEQ l−1. For solid samples, HRGC/MS‐TEQ values ranged from 3.44 to 0.49 ng TEQ g−1 and correlated less well than liquid samples (correlation coefficient 0.64) with values obtained by EROD‐TEQ ranging from 2.27 to 0.93 ng TEQ g−1. The overestimation of RTG‐2 EROD‐TEQ (1.2 ± 0.92 of values established by HRGC/MS) and the absence of false‐negative results may limit analytical costs by eliminating the need for follow‐up GC/MS analysis on the negative samples. We suggest that RTG‐2 EROD bioassay is an inexpensive means for preliminary dioxin and furan positive screenings of waste samples. Copyright © 2010 John Wiley & Sons, Ltd. Determination of dioxin‐like compounds using in vitro EROD bioassays is important because it detects all AhR ligands. This work compares RTG‐2 cell line EROD bioassay with HRGC/MS for assessing samples contaminated with dioxin‐like compounds. The correlation coefficient values, comparing HRGC/MS‐TEQ and EROD‐TEQ, were better for liquid samples (0.99) than for solid samples (0.64). The overestimation of RTG‐2 EROD‐TEQ (1.2 ± 0.92 of values established by HRGC/MS) and the absence of false‐negatives results, may limit analytical costs on the negative samples.