Separation, identification and quantification of tetrodotoxin and its analogs by LC–MS without calibration of individual analogs

• Published in: Toxicon (Oxford) Vol. 57; no. 6; pp. 938 - 943
• Main Authors: Chen, Xiao-Wu, Liu, Hong-Xia, Jin, Yi-Bao, Li, Shang-Fu, Bi, Xin, Chung, Stephen, Zhang, Shu-Sheng, Jiang, Yu-Yang
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.05.2011; Elsevier
• Subjects: Animal poisons toxicology. Antivenoms; Biological and medical sciences; Chromatography, Liquid - methods; detection limit; fish; Liquid chromatography–mass spectrometry (LC–MS); Medical sciences; Molecular Structure; rapid methods; Tandem Mass Spectrometry - methods; tetrodotoxin; Tetrodotoxin - analysis; Tetrodotoxin - chemistry; Tetrodotoxin - isolation & purification; Tetrodotoxin and its analogs; Toxicology; Without calibration factor; Tetrodotoxin and its analogs; Liquid chromatography–mass spectrometry (LC–MS); Without calibration factor; Human; Liquid chromatography-mass spectrometry (LC-MS); Analog; Liquid chromatography; Identification; Calibration; Mass spectrometry; Quantitative analysis
• ISSN: 0041-0101; 1879-3150; 1879-3150
• DOI: 10.1016/j.toxicon.2011.03.011

We report here a simple and rapid method of separation, identification and quantification of tetrodotoxin (TTX) and its analogs in partially purified extract by LC–MS. Except for the main component calibration of individual components was not necessary. TTX and four of its analogs in the puffer fish extract were identified and quantified. The limits of detection and quantification, the linear range and accuracy of the protocol were validated.