Effects of corn steep liquor on β-poly(l-malic acid) production in Aureobasidium melanogenum

• Published in: AMB Express Vol. 10; no. 1; p. 211
• Main Authors: Wang, Genan, Shi, Bingyi, Zhang, Pan, Zhao, Tingbin, Yin, Haisong, Qiao, Changsheng
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2020; Springer Nature B.V; SpringerOpen
• Subjects: Amino acids; Aureobasidium melanogenum; Biomedical and Life Sciences; Biopolymers; Biosynthesis; Biotechnology; Cell growth; Corn; Corn steep liquor; Food industry; Growth factors; Intermediates; Life Sciences; Low level; Malic acid; Metabolites; Metabolomics; Microbial Genetics and Genomics; Microbiology; Microorganisms; Nutritive value; Organic acids; Original; Original Article; Tricarboxylic acid cycle; Tyrosine; β-poly(l-malic acid) (PMLA); β-poly; Metabolomics; malic acid) (PMLA; Corn steep liquor
• ISSN: 2191-0855; 2191-0855
• DOI: 10.1186/s13568-020-01147-8

β-poly( l -malic acid) (PMLA) is a water-soluble biopolymer used in medicine, food, and other industries. However, the low level of PMLA biosynthesis in microorganisms limits its further application in the biotechnological industry. In this study, corn steep liquor (CSL), which processes high nutritional value and low-cost characteristics, was selected as a growth factor to increase the PMLA production in strain, Aureobasidium melanogenum , and its metabolomics change under the CSL addition was investigated. The results indicated that, with 3 g/L CSL, PMLA production, cell growth, and yield (Y p/x ) were increased by 32.76%, 41.82%, and 47.43%, respectively. The intracellular metabolites of A. melanogenum , such as amino acids, organic acids, and key intermediates in the TCA cycle, increased after the addition of CSL, and the enrichment analysis showed that tyrosine may play a major role in the PMLA biosynthesis. The results presented in this study demonstrated that the addition of CSL would be an efficient approach to improve PMLA production.