Growth and site-specific organization of micron-scale biomolecular devices on living mammalian cells

• Published in: Nature communications Vol. 12; no. 1; p. 5729
• Main Authors: Jia, Sisi, Phua, Siew Cheng, Nihongaki, Yuta, Li, Yizeng, Pacella, Michael, Li, Yi, Mohammed, Abdul M., Sun, Sean, Inoue, Takanari, Schulman, Rebecca
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 30.09.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 13/31; 14; 14/1; 14/19; 14/34; 14/35; 14/63; 45; 639/301/357/341; 639/925/926/1048; Amplification; Antibodies; Assemblies; Binding sites; Biology; Cell signaling; Cell surface; Cilia; Deoxyribonucleic acid; DNA; Fabrication; Filaments; Filopodia; Humanities and Social Sciences; Information processing; Mammalian cells; Mammals; Measuring instruments; Mechanical engineering; Mesoscale phenomena; multidisciplinary; Nanoparticles; Nanotechnology; Nanotubes; Peptides; Proteins; Science; Science (multidisciplinary); Seeds; Self-assembly; Sensors; Shear stress
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-25890-z

Mesoscale molecular assemblies on the cell surface, such as cilia and filopodia, integrate information, control transport and amplify signals. Designer cell-surface assemblies could control these cellular functions. Such assemblies could be constructed from synthetic components ex vivo, making it possible to form such structures using modern nanoscale self-assembly and fabrication techniques, and then oriented on the cell surface. Here we integrate synthetic devices, micron-scale DNA nanotubes, with mammalian cells by anchoring them by their ends to specific cell surface receptors. These filaments can measure shear stresses between 0-2 dyn/cm 2 , a regime important for cell signaling. Nanotubes can also grow while anchored to cells, thus acting as dynamic cell components. This approach to cell surface engineering, in which synthetic biomolecular assemblies are organized with existing cellular architecture, could make it possible to build new types of sensors, machines and scaffolds that can interface with, control and measure properties of cells. Mesoscale molecular assemblies on the cell surface integrate information and amplify signals. Here the authors integrate DNA nanotubes in a controlled manner with mammalian cells to act as sheer stress meters.