Rapid detection of important human pathogenic Phleboviruses

• Published in: Journal of clinical virology Vol. 41; no. 2; pp. 138 - 142
• Main Authors: Weidmann, Manfred, Sanchez-Seco, M. Paz, Sall, Amadou A., Ly, Peinda Ogo, Thiongane, Yaya, Lô, Modou M., Schley, Heike, Hufert, Frank T.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.02.2008; Elsevier Science
• Subjects: Allergy and Immunology; Animals; Biological and medical sciences; Bunyaviridae; Bunyaviridae Infections - virology; Bunyavirus; Cercopithecus aethiops; Fundamental and applied biological sciences. Psychology; Human viral diseases; Humans; Infectious Disease; Infectious diseases; Lightcycler; Medical sciences; Microbiology; Miscellaneous; Phlebovirus; Phlebovirus - classification; Phlebovirus - genetics; Phlebovirus - isolation & purification; Real-time PCR; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Reverse Transcriptase Polymerase Chain Reaction - methods; Rift Valley Fever virus; Rift Valley fever virus - genetics; Rift Valley fever virus - isolation & purification; Sandfly fever Naples virus; Sandfly fever Naples virus - genetics; Sandfly fever Naples virus - isolation & purification; Sandfly fever Sicilian virus; Sensitivity and Specificity; Smartcycler; Species Specificity; Taq Polymerase - metabolism; Taqman; Time Factors; Toscana virus; Vero Cells; Viral diseases; Virology; Lightcycler; Taqman; Real-time PCR; Bunyavirus; Toscana virus; Rift Valley Fever virus; Smartcycler; Phlebovirus; Human; Microbiology; Sandfly fever group virus; Zoopathogen; Real time; Rift valley fever virus; Virology; Virus; Pathogenicity; Polymerase chain reaction; Bunyaviridae; Toscana vims; Detection
• ISSN: 1386-6532; 1873-5967
• DOI: 10.1016/j.jcv.2007.10.001

Rapid diagnostics are not available for several human pathogens in the genus Phlebovirus of the Bunyaviridae. To develop RT-PCR assays for Sandfly Fever Sicilian virus (SFSV), Sandfly Fever Naples virus (SFNV), Toscana virus (TOSV) and Rift Valley Fever virus (RVFV). RNA standards were generated and used to test the performance of the assays. A detection limit of 10–100 RNA molecules was determined for the SFSV, TOSV and RVFV assays. The sensitivity of the SFNV assay was not determined. The TOSV and the RVFV assays detected recent isolates from Spain and Africa, respectively. The assays should help to improve surveillance of pathogenic Phleboviruses.